Growth of Human Mast Cells from Bone Marrow and Peripheral Blood-derived Cd34+ Pluripotent Progenitor Cells.
From: Laboratory of Allergic Diseases, Mast Cell Biology Section, NIH/NIAID, Bethesda, MD, USA.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 315
- Issue:
- Pages: 105-12
- Medium: Print
- Language: English
- Citation (JAMA): Kirshenbaum Arnold S, Metcalfe Dean D, et al. Growth of Human Mast Cells from Bone Marrow and Peripheral Blood-derived Cd34+ Pluripotent Progenitor Cells.. Methods Mol. Biol. 2006;315:105-12
Abstract
Human mast cells (HMCs) are derived from a CD34+ pluripotent progenitor cell that is Kit (CD117+), CD13+, Fc epsilonRI- and lacks lineage-specific surface markers. Bone marrow and peripheral blood are two tissue sources available for obtaining CD34+ progenitor cells from which to culture HMCs. CD34+ cells can be isolated and enriched by magnetic separation columns and stored under specific conditions until ready for use. Alternatively, enriched CD34+ cells may be immediately cultured in serum-free culture media containing recombinant human stem cell factor (rhSCF), rhIL-6, and rhIL-3 (first week only). Weekly hemidepletions and the removal of adherent cells and/or debris enables the investigator to obtain HMC cultures, identified by Wright-Giemsa and acidic toluidine blue stains, by 8-10 wk.
Mesh Headings (Keywords): Antigens, CD34, Biological Markers, Bone Marrow Cells, Cells, Cultured, Humans, Immunomagnetic Separation, Leukocytes, Mast Cells, Pluripotent Stem Cells
Check for Full Text / PubMed Unique Identifier (PMID): 16110152
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