Isolation, Culture, and Characterization of Intestinal Mast Cells.
From: Department of Cell Biology and Infection, Institute Pasteur, Paris, France.
Methods in molecular biology (Clifton, N.J.)
- Publish Date: 2006
- ISSN: 1064-3745
- Volume: 315
- Issue:
- Pages: 123-38
- Medium: Print
- Language: English
- Citation (JAMA): Sellge Gernot, Bischoff Stephan C, et al. Isolation, Culture, and Characterization of Intestinal Mast Cells.. Methods Mol. Biol. 2006;315:123-38
Abstract
Mast cells are bone-marrow-derived tissue cells typically located at barrier sites of the body, such as skin, mucosal barriers, or blood barriers, that is, around blood vessels. This location suggests that mast cells might have a function as immunological “gate-keepers” or “watch dogs” and, indeed, some recent functional data support this idea. Mast cells derive from myeloid progenitors, but in contrast to other myeloid cells, they leave the bone marrow in an immature state; therefore, mast cells are not found in the blood under normal conditions. For full maturation, the tissue environment is necessary. Thus, mature mast cells can be only isolated from tissue such as skin or mucosal sites, which makes mast cell isolation rather complicated. Alternatively, mast cell progenitors can be isolated from the bone marrow, peripheral blood, or cord blood, which is easier but requires subsequent in vitro maturation of mast cells as far as possible using cytokines. This chapter describes a rather new technique of mast cell isolation from human intestinal mucosal tissue yielding approx 1-5 million pure and viable human mast cells suitable to perform functional and cell culture experiments.
Mesh Headings (Keywords): Animals, Cell Culture Techniques, Cells, Cultured, Cytokines, Humans, Immunomagnetic Separation, Intestinal Mucosa, Mast Cells, Stem Cell Factor
Check for Full Text / PubMed Unique Identifier (PMID): 16110154
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