Expressions of Bovine Ifn-gamma and Foot-and-mouth Disease Vp1 Antigen in P. Pastoris and Their Effects on Mouse Immune Response to Fmd Antigens.
From: College of Veterinary Medicine, No. 2, yuanmingyuan west Road, haidian District 100094, Beijing, PR China. shixj@bjciq.gov.cn
Vaccine
- Publish Date: Jan 2006
- ISSN: 0264-410X
- Volume: 24
- Issue: 1
- Pages: 82-9
- Medium: Print
- Language: English
- Citation (JAMA): Shi Xi-Ju, Wang Bin, Zhang Can, et al. Expressions of Bovine Ifn-gamma and Foot-and-mouth Disease Vp1 Antigen in P. Pastoris and Their Effects on Mouse Immune Response to Fmd Antigens.. Vaccine Jan 2006;24:82-9
Abstract
As a highly contagious disease in cloven-hoofed animals, foot-and-mouth disease virus (FMDV) may cause a considerable social-economic loss in those countries affected. IFN-gamma has a wide range of antiviral and immune modulating functions. Thus, to study the immune enhancing effects of recombinant Bovine IFN-gamma (rBoIFN-gamma) on a recombinant FMDV vaccine, BoIFN-gamma, FMDV VP1 and BoIFN-gamma/VP fusion genes were cloned, expressed, co-expressed in pichia pastoris (P. pastoris) respectively, and subsequent immune effects have been evaluated in this study. The results showed that the genes encoding for BoIFN-gamma, VP1 and BoIFN-gamma/VP1 are successfully expressed in P. pastoris and their products are directly secreted into the cultural supernatant at a high level of 1.0 g/L analyzed by thin-layer scanning. In addition, rVP1 alone could induce both humoral and marginal cell-mediated immune responses in mice, while the group with co-inoculations of rBoIFN-gamma could markedly enhance both humoral and cell-mediated immune responses; even more dramatic immune responses were observed with the group inoculated with the fusion product, rBoIFN-gamma/VP1. The fusion product could be further investigated for its utility of FMDV vaccine development.
Mesh Headings (Keywords): Animals, Antibodies, Viral, Capsid Proteins, Cattle, Cytokines, Female, Foot-and-Mouth Disease, Foot-and-Mouth Disease Virus, Immunization, Immunoglobulin G, Interferon Type II, Lymphocyte Activation, Mice, Mice, Inbred BALB C, Pichia, Plasmids, Recombinant Fusion Proteins, Reverse Transcriptase Polymerase Chain Reaction, T-Lymphocytes, Vaccines, Synthetic, Viral Vaccines
Check for Full Text / PubMed Unique Identifier (PMID): 16135391
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.