The Use of Th1 Cytokines, Il-12 and Il-23, to Modulate the Immune Response Raised to a Dna Vaccine Delivered by Gene Gun.
From: Department of Microbiology and Immunology, University of Otago, PO Box 56, Dunedin, New Zealand.
Vaccine
- Publish Date: May 2006
- ISSN: 0264-410X
- Volume: 24
- Issue: 21
- Pages: 4471-4
- Medium: Print
- Language: English
- Citation (JAMA): Williman Jonathan, Lockhart Euan, Slobbe Lynn, et al. The Use of Th1 Cytokines, Il-12 and Il-23, to Modulate the Immune Response Raised to a Dna Vaccine Delivered by Gene Gun.. Vaccine May 2006;24:4471-4
Abstract
Unlike intramuscular injection, gene gun delivery of DNA drives a strong type 2 response. In an effort to counter this, we have genetically fused the type 1 cytokines, IL-12 and IL-23, to the hemagglutinin (HA) gene from influenza APR/8/34, and delivered these DNA constructs to Balb/c mice. Gene gun delivery of the HA gene was able to induce antibody production by all vaccinated mice. Linking of IL-12 caused almost complete suppression of immune responses whereas mice vaccinated with IL-23HA showed long-lived IgG1 antibody levels. Splenocytes from IL-23HA vaccinated mice also tended to produce more IL-5 and IFNgamma after restimulation in vitro than splenocytes from HA vaccinated mice. While codelivery of IL-23 did not change the type of immune response it may increase its longevity following vaccination.
Mesh Headings (Keywords): Animals, Antibodies, Viral, Base Sequence, Biolistics, Cell Line, DNA Primers, Enzyme-Linked Immunosorbent Assay, Humans, Interleukin-12, Interleukin-23, Mice, Orthomyxoviridae, Th1 Cells, Vaccines, DNA
Check for Full Text / PubMed Unique Identifier (PMID): 16140432
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