Cell Adhesion on a Polymerized Peptide-amphiphile Monolayer.
From: Department of Chemical Engineering, Materials Research Laboratory (MRL) and the Institute for Collaborative Biotechnologies, University of California at Santa Barbara, Santa Barbara, CA 93106, USA. biesalsk@imtek.de
Biomaterials
- Publish Date: Mar 2006
- ISSN: 0142-9612
- Volume: 27
- Issue: 8
- Pages: 1259-69
- Medium: Print
- Language: English
- Citation (JAMA): Biesalski Markus A, Knaebel Alexandra, Tu Raymond, et al. Cell Adhesion on a Polymerized Peptide-amphiphile Monolayer.. Biomaterials Mar 2006;27:1259-69
Abstract
We report the synthesis and characterization of a stable polymerized monolayer of peptide-amphihiles on a planar solid support that promotes mouse fibroblast cell adhesion and spreading. Peptide-amphiphiles consisting of a polymerizable fatty acid attached to a short RGD containing peptide sequence are self-assembled and polymerized at the water-air interface by means of the Langmuir- Blodgett technique. The surface concentration of the peptide-amphiphile is varied by co-spreading the peptide-amphiphile with an analogous non-modified polymerizable amphiphile at the water/air interface, prior to UV light-induced polymerization. The polymerized monolayer is transferred onto a hydrophobized smooth mica surface and the resulting surfaces have been investigated with respect to directing the cell adhesion and spreading of mouse fibroblast cells in a serum-free medium. Fibroblast cells adhere and spread on surfaces exposing the bioactive ligand but do not spread on reference surfaces without peptide. We find a maximum number of adherent cells at rather high peptide surface concentrations of about 10 mol% in the mixed monolayer, equivalent to more than 50 pmol/cm2 peptide on the surface of the film. We attribute this finding to a limited accessibility of the ligands by the integrins. Because of the stability of the polymerized peptide-amphiphile monolayer, these surfaces can be re-seeded multiple times with cells, i.e. adherent cells can be removed from the surface, the surface can be sterilized and cells can be re-attached.
Mesh Headings (Keywords): Aluminum Silicates, Animals, Cell Adhesion, Cells, Cultured, Fibroblasts, Mice, Microscopy, Atomic Force, Oligopeptides, Surface-Active Agents
Check for Full Text / PubMed Unique Identifier (PMID): 16157369
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