Vasorelaxant Effect of the Flavonoid Galangin on Isolated Rat Thoracic Aorta.
From: Dipartimento di Farmacologia Sperimentale, Università degli Studi di Napoli Federico II, via D. Montesano 49, 80131 Napoli, Italy.
Life sciences
- Publish Date: Jan 2006
- ISSN: 0024-3205
- Volume: 78
- Issue: 8
- Pages: 825-30
- Medium: Print
- Language: English
- Citation (JAMA): Morello Silvana, Vellecco Valentina, Alfieri Alessio, et al. Vasorelaxant Effect of the Flavonoid Galangin on Isolated Rat Thoracic Aorta.. Life Sci. Jan 2006;78:825-30
Abstract
Here we investigated the effect of the flavonoid galangin in isolated rat thoracic aortic rings. Galangin (0.1-100 microM) induced relaxation in rings pre-contracted with phenylephrine (PE 1 microM) or with KCl (100 mM) or pre-treated with the nitric oxide synthase inhibitor Nomega-nitro-L-arginine methyl ester (L-NAME, 100 microM), the cyclooxygenase inhibitor indomethacin (10 microM) and the adenylate cyclase inhibitor, SQ 22,536 (100 microM). In another set of experiments, rat aortic rings were incubated with galangin (1-100 microM) and the contractile responses to PE (0.001-3 microM) or to KCl (60 mM) were evaluated. We also evaluated the effect of galangin (100 microM) on PE (10 microM)-induced contraction in a Ca2+-free medium. Galangin relaxed aortic rings with or without endothelium. Galangin effect was significantly inhibited by L-NAME. Galangin inhibited the contractile response to PE, either in presence or in absence of external calcium, and to KCl. In the end, we also found that galangin caused nitric oxide (NO) release from aortic rings and abolished the increase in [Ca2+]i triggered by PE or KCl in aortic smooth muscle cells, either in presence and in absence of external Ca2+. Our results suggest that galangin reduces the contractility of rat aortic rings through an endothelium-dependent mechanism, involving NO, and also through an endothelium-independent mechanism, inhibiting calcium movements through cell membranes.
Mesh Headings (Keywords): Adenine, Animals, Aorta, Thoracic, Calcium, Dose-Response Relationship, Drug, Drug Combinations, Flavonoids, Indomethacin, Male, NG-Nitroarginine Methyl Ester, Nitric Oxide, Phenylephrine, Potassium Chloride, Propolis, Rats, Rats, Wistar, Vasodilation, Vasodilator Agents
Check for Full Text / PubMed Unique Identifier (PMID): 16169019
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