Oxidized Phospatidylcholine but Not Native Phosphatidylcholine Inhibits Inducible Nitric Oxide Synthase in Raw 264.7 Macrophages.
From: Medical University of Vienna, Institute of Physiology, Schwarzspanierstrasse 17, A-1090 Vienna, Austria. roswitha.friedl@univie.ac.at
Life sciences
- Publish Date: Feb 2006
- ISSN: 0024-3205
- Volume: 78
- Issue: 14
- Pages: 1586-91
- Medium: Print
- Language: English
- Citation (JAMA): Friedl Roswitha, Pichler Ingeborg, Spieckermann Paul, et al. Oxidized Phospatidylcholine but Not Native Phosphatidylcholine Inhibits Inducible Nitric Oxide Synthase in Raw 264.7 Macrophages.. Life Sci. Feb 2006;78:1586-91
Abstract
This study was designed to compare the effects of oxidized 1-palmitoyl-2-arachidonyl-sn-glycero-3-phosphorylcholine (PAPC) and native PAPC on the inducible nitric oxide synthase (iNOS) in the macrophage cell line RAW 264.7. Macrophages stimulated by bacterial lipopolysaccharide (1 microg/ml) were incubated with increasing amounts of native or oxidized PAPC (oxPAPC, 10-20 microg/ml). Cells incubated with oxPAPC showed a dose-dependent inhibition of inducible nitric oxide synthesis, as well as reduced iNOS protein expression and mRNA levels. Additionally, chromatin immunoprecipitation assay revealed that oxPAPC reduced the interaction of the active NF-kappaB subunit p65 with the iNOS promoter region when compared to native PAPC.
Mesh Headings (Keywords): Animals, Cells, Cultured, Chromatin Immunoprecipitation, Lipopolysaccharides, Macrophages, Mice, Nitric Oxide Synthase Type II, Oxidation-Reduction, Phospholipid Ethers, Promoter Regions (Genetics), Transcription Factor RelA
Check for Full Text / PubMed Unique Identifier (PMID): 16242732
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