Ca2+-independent Binding and Cellular Expression Profiles Question a Significant Role of Calmyrin in Transduction of Ca2+-signals to Alzheimer's Disease-related Presenilin 2 in Forebrain.
From: Laboratory of Neurodegeneration, International Institute of Molecular and Cell Biology, Trojdena 4, 02-109 Warsaw, Poland.
Biochimica et biophysica acta
- Publish Date: Jan 2006
- ISSN: 0006-3002
- Volume: 1762
- Issue: 1
- Pages: 66-72
- Medium: Print
- Language: English
- Citation (JAMA): Blazejczyk Magdalena, Wojda Urszula, Sobczak Adam, et al. Ca2+-independent Binding and Cellular Expression Profiles Question a Significant Role of Calmyrin in Transduction of Ca2+-signals to Alzheimer's Disease-related Presenilin 2 in Forebrain.. Biochim. Biophys. Acta Jan 2006;1762:66-72
Abstract
The interaction between the EF-hand Ca(2+)-binding protein calmyrin and presenilin 2 (PS2) has been suggested to play a role in Alzheimer’s disease (AD). We now report that calmyrin binds specifically endogenous PS2 and not PS1. However, binding appears to be Ca(2+)-independent and calmyrin does not exhibit a Ca(2+)-dependent translocation to intracellular membranes as demonstrated in a Ca(2+)-myristoyl switch assay. Moreover, calmyrin is only present at very low levels in brain areas associated with the onset of AD. In rat, forebrain calmyrin is localized only in a subset of principal neurons, similarly as in human forebrain. Finally, subcellular fractionation demonstrates only a limited overlap of calmyrin and PS2 at neuronal membranes. We therefore conclude that calmyrin will not contribute significantly as a Ca(2+)-sensor that transduces Ca(2+)-signaling events to PS2 in forebrain.
Mesh Headings (Keywords): Alzheimer Disease, Animals, COS Cells, Calcium, Calcium Signaling, Calcium-Binding Proteins, Cercopithecus aethiops, Gene Expression Profiling, Hela Cells, Humans, Intracellular Membranes, Membrane Proteins, Presenilin-2, Prosencephalon, Protein Binding, Rats, Rats, Wistar, Recombinant Fusion Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16257512
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