A Toxoplasma Gondii Phosphoinositide Phospholipase C (Tgpi-plc) with High Affinity for Phosphatidylinositol.
From: Department of Cellular Biology, and Center for Tropical and Emerging Global Diseases, The University of Georgia, Athens, GA 30602, USA.
The Biochemical journal
- Publish Date: Mar 2006
- ISSN: 1470-8728
- Volume: 394
- Issue: Pt 2
- Pages: 417-25
- Medium: Internet
- Language: English
- Citation (JAMA): Fang Jianmin, Marchesini Norma, Moreno Silvia N J, et al. A Toxoplasma Gondii Phosphoinositide Phospholipase C (Tgpi-plc) with High Affinity for Phosphatidylinositol.. Biochem. J. Mar 2006;394:417-25
Abstract
The Toxoplasma gondii phosphoinositide-specific phospholipase C gene (TgPI-PLC) was cloned, sequenced and expressed in Escherichia coli and its enzymatic characteristics were investigated. TgPI-PLC is present in the genome as a single-copy gene consisting of 22 exons interrupted by 21 introns, and encodes a polypeptide of 1097 amino acids with a predicted molecular mass of 121 kDa. In addition to the conserved catalytic X and Y domains, TgPI-PLC contains an apparent N-terminal PH domain, an EF hand motif and a C-terminal C2 domain. When compared with mammalian delta-type PI-PLC, TgPI-PLC has an additional extended N-terminus and two insertions in the region between the X and Y domains, with a 31-35% identity over the whole sequence. Recombinant TgPI-PLC, as well as the native enzyme obtained from crude membrane extracts of the parasite, was more active with phosphatidylinositol than with phosphatidylinositol 4,5-bisphosphate as substrate. Indirect immunofluorescence analysis using an affinity-purified antibody against TgPI-PLC revealed that this enzyme localizes in the plasma membrane of the parasites.
Mesh Headings (Keywords): Amino Acid Sequence, Animals, Calcium, Enzyme Stability, Gene Expression Regulation, Hydrogen-Ion Concentration, Magnesium, Molecular Sequence Data, Phosphatidylinositol Diacylglycerol-Lyase, Phosphatidylinositols, Phosphoinositide Phospholipase C, Protein Transport, Substrate Specificity, Temperature, Toxoplasma
Check for Full Text / PubMed Unique Identifier (PMID): 16288600
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