Translational Enhancement of Hcv Rna Genotype 1b by 3'-untranslated and Envelope 2 Protein-coding Sequences.
From: The Second Department of Internal Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan.
Virology
- Publish Date: Feb 2006
- ISSN: 0042-6822
- Volume: 345
- Issue: 2
- Pages: 404-15
- Medium: Print
- Language: English
- Citation (JAMA): Morikawa Kenichi, Ito Takayoshi, Nozawa Hisako, et al. Translational Enhancement of Hcv Rna Genotype 1b by 3'-untranslated and Envelope 2 Protein-coding Sequences.. Virology Feb 2006;345:404-15
Abstract
HCV RNA has a unique regulatory mechanism for translation. The X region of 3’-UTR and core-coding sequence regulate HCV translation. In this study, we clarified that the entire 3’-UTR also enhances HCV translation, and the envelope-coding sequence of HCV genotype 1b increases degree of this enhancement. In the luciferase reporter assay using rabbit reticulocyte lysates, translational enhancement by 3’-UTR with core to E2 regions was 25-fold higher when compared with control RNA lacking the 3’-UTR. Presence of the entire E2 sequence was important for this enhancement. This phenomenon was not due to transcript stability, and envelope protein alone did not affect translation. E2-coding sequence of genotype 1a had no effect on translation. We observed the same results in animal cell culture systems using bicistronic RNA. Structural protein-coding sequences and 3’-UTR of HCV RNA regulate viral translation, and a target for antiviral agents may be present in these regions.
Mesh Headings (Keywords): 3’ Untranslated Regions, Animals, Base Sequence, Cell Line, Gene Expression Regulation, Viral, Genotype, Hepacivirus, Humans, Molecular Sequence Data, Protein Biosynthesis, RNA, Viral, Rabbits, Reticulocytes, Ribosomes, Viral Envelope Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16289655
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