Anti-inflammatory Effects of Il-4 and Dynamic Compression in Il-1beta Stimulated Chondrocytes.
From: Department of Engineering, Queen Mary, University of London, Mile End Road, London E1 4NS, UK. T.T.Chowdhury@qmul.ac.uk
Biochemical and biophysical research communications
- Publish Date: Jan 2006
- ISSN: 0006-291X
- Volume: 339
- Issue: 1
- Pages: 241-7
- Medium: Print
- Language: English
- Citation (JAMA): Chowdhury T T, Bader D L, Lee D A, et al. Anti-inflammatory Effects of Il-4 and Dynamic Compression in Il-1beta Stimulated Chondrocytes.. Biochem. Biophys. Res. Commun. Jan 2006;339:241-7
Abstract
Mechanical loading can counteract inflammatory pathways induced by IL-1beta by inhibiting *NO and PGE2, catabolic mediators known to be involved in cartilage degradation. The current study investigates the potential of dynamic compression, in combination with the anti-inflammatory cytokine, IL-4, to further abrogate the IL-1beta induced effects. The data presented demonstrate that IL-4 alone can inhibit nitrite release in the presence and absence of IL-1beta and partially reverse the IL-1beta induced PGE2 release. When provided in combination, IL-4 and dynamic compression could further abrogate the IL-1beta induced nitrite and PGE2 release. IL-1beta inhibited [3H]thymidine incorporation and this effect could be reversed by IL-4 or dynamic strain alone or both in combination. By contrast, 35SO4 incorporation was not influenced by IL-4 and/or dynamic strain in IL-1beta stimulated constructs. IL-4 and mechanical loading may therefore provide a potential protective mechanism for cartilage destruction as observed in OA.
Mesh Headings (Keywords): Animals, Cattle, Cells, Cultured, Chondrocytes, Dinoprostone, Interleukin-1, Interleukin-4, Mechanotransduction, Cellular, Nitrogen Oxides, Stress, Mechanical
Check for Full Text / PubMed Unique Identifier (PMID): 16297873
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