Stem Cell Mobilization by Hyperbaric Oxygen.
From: Institute for Environmental Medicine, University of Pennsylvania, Philadelphia, PA 19104-6068, USA. sthom@mail.med.upenn.edu
American journal of physiology. Heart and circulatory physiology
- Publish Date: Apr 2006
- ISSN: 0363-6135
- Volume: 290
- Issue: 4
- Pages: H1378-86
- Medium: Print
- Language: English
- Citation (JAMA): Thom Stephen R, Bhopale Veena M, Velazquez Omaida C, et al. Stem Cell Mobilization by Hyperbaric Oxygen.. Am. J. Physiol. Heart Circ. Physiol. Apr 2006;290:H1378-86
Abstract
We hypothesized that exposure to hyperbaric oxygen (HBO(2)) would mobilize stem/progenitor cells from the bone marrow by a nitric oxide (*NO) -dependent mechanism. The population of CD34(+) cells in the peripheral circulation of humans doubled in response to a single exposure to 2.0 atmospheres absolute (ATA) O(2) for 2 h. Over a course of 20 treatments, circulating CD34(+) cells increased eightfold, although the overall circulating white cell count was not significantly increased. The number of colony-forming cells (CFCs) increased from 16 +/- 2 to 26 +/- 3 CFCs/100,000 monocytes plated. Elevations in CFCs were entirely due to the CD34(+) subpopulation, but increased cell growth only occurred in samples obtained immediately posttreatment. A high proportion of progeny cells express receptors for vascular endothelial growth factor-2 and for stromal-derived growth factor. In mice, HBO(2) increased circulating stem cell factor by 50%, increased the number of circulating cells expressing stem cell antigen-1 and CD34 by 3.4-fold, and doubled the number of CFCs. Bone marrow *NO concentration increased by 1,008 +/- 255 nM in association with HBO(2). Stem cell mobilization did not occur in knockout mice lacking genes for endothelial *NO synthase. Moreover, pretreatment of wild-type mice with a *NO synthase inhibitor prevented the HBO(2)-induced elevation in stem cell factor and circulating stem cells. We conclude that HBO(2) mobilizes stem/progenitor cells by stimulating *NO synthesis.
Mesh Headings (Keywords): Animals, Antigens, CD34, Cell Movement, Cell Proliferation, Cells, Cultured, Female, Hematopoietic Stem Cells, Humans, Hyperbaric Oxygenation, Male, Mice, Mice, Knockout, Middle Aged, Nitric Oxide, Oxygen
Check for Full Text / PubMed Unique Identifier (PMID): 16299259
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