F-spondin Promotes Nerve Precursor Differentiation.
From: The Salk Institute, La Jolla, California 92037, USA. Schubert@salk.edu
Journal of neurochemistry
- Publish Date: Jan 2006
- ISSN: 0022-3042
- Volume: 96
- Issue: 2
- Pages: 444-53
- Medium: Print
- Language: English
- Citation (JAMA): Schubert David, Klar Avihu, Park Minkyu, et al. F-spondin Promotes Nerve Precursor Differentiation.. J. Neurochem. Jan 2006;96:444-53
Abstract
Cells in the developing nervous system secrete a large number of proteins that regulate the migration and differentiation of their neighbors. It is shown here that a clonal central nervous system cell line secretes a protein that causes both a rat hippocampal progenitor cell line and primary cortical neural cells to differentiate into cells with the morphological and biochemical features of neurons. This protein was identified as F-spondin. Analysis of F-spondin isoforms secreted from transfected cells shows that the core protein without the thrombospondin type 1 repeats is sufficient to promote neuronal differentiation when adsorbed to a surface. F-spondin can also inhibit neurite outgrowth while allowing the expression of nerve-specific proteins when present in a soluble form at high concentrations. Therefore, F-spondin can alter cell differentiation in multiple ways, depending upon its concentration and distribution between substrate-attached and soluble forms.
Mesh Headings (Keywords): Animals, Cell Differentiation, Cell Line, Transformed, Cells, Cultured, Central Nervous System, Cerebral Cortex, Hippocampus, Nerve Tissue Proteins, Neural Inhibition, Neurites, Neurons, Peptides, Rats, Stem Cells, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 16300627
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