Cystatin C Expression is Increased in the Hippocampus Following Photothrombotic Stroke in Rat.
From: A.I. Virtanen Institute for Molecular Sciences, University of Kuopio, P.O. Box 1627, FIN-70211 Kuopio, Finland.
Neuroscience letters
- Publish Date: Mar 2006
- ISSN: 0304-3940
- Volume: 395
- Issue: 2
- Pages: 108-13
- Medium: Print
- Language: English
- Citation (JAMA): Pirttilä Terhi J, Pitkänen Asla, et al. Cystatin C Expression is Increased in the Hippocampus Following Photothrombotic Stroke in Rat.. Neurosci. Lett. Mar 2006;395:108-13
Abstract
Stroke is a major cause of epilepsy, but the molecular mechanisms underlying post-stroke epileptogenesis are unknown. The expression of cystatin C, a cysteine protease inhibitor, is increased in the hippocampus during status epilepticus (SE)-induced epileptogenesis, and regulates both cell death and birth. To test the hypothesis that increased cystatin C expression represents a common molecular alteration induced by epileptogenic brain insults, we investigated the time course, cellular localization, and association of cystatin C expression with neuronal damage during post-stroke epileptogenesis. Stroke was induced with photothrombosis, which leads to epilepsy in approximately 20-30% of rats. Cystatin C expression was increased in the CA1 area of the hippocampus 4 days after photothrombosis, when the diameter of the lesion was the largest. Double-labeling and confocal analysis indicated that cystatin C was expressed in astrocytes and microglia. Unlike after SE, cystatin C expression did not change in the dentate gyrus. Also, increased cystatin C expression was not associated with neurodegeneration, which was demonstrated as an absence of Fluoro Jade B-positive cells in adjacent sections. The present study provides evidence that cystatin C may be involved in cellular alterations that occur after an epileptogenic insult, not only after SE but also after photothrombotic stroke.
Mesh Headings (Keywords): Animals, Cystatins, Fluorescent Dyes, Hippocampus, Male, Microscopy, Confocal, Nerve Degeneration, Neuroglia, Rats, Rats, Sprague-Dawley, Rose Bengal, Status Epilepticus, Stroke
Check for Full Text / PubMed Unique Identifier (PMID): 16309830
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