Anti-apoptotic and Anti-senescence Effects of Klotho on Vascular Endothelial Cells.
From: Department of Geriatric Medicine, Osaka University Graduate School of Medicine, Suita, Japan.
Biochemical and biophysical research communications
- Publish Date: Jan 2006
- ISSN: 0006-291X
- Volume: 339
- Issue: 3
- Pages: 827-32
- Medium: Print
- Language: English
- Citation (JAMA): Ikushima Masashi, Rakugi Hiromi, Ishikawa Kazuhiko, et al. Anti-apoptotic and Anti-senescence Effects of Klotho on Vascular Endothelial Cells.. Biochem. Biophys. Res. Commun. Jan 2006;339:827-32
Abstract
Klotho-mutated mice manifest multiple age-related disorders that are observed in humans. A recent study suggested that Klotho protein might function as an anti-aging hormone in mammals. Because it has been reported that apoptosis and senescence in vascular endothelial cells are closely related to the progression of atherosclerosis, we investigated Klotho’s ability to interfere with apoptosis and cellular senescence in human umbilical vascular endothelial cells (HUVEC). Klotho overexpression decreased H(2)O(2)-induced apoptosis in COS-1 cells and Jurkat cells. Klotho protein also reduced H(2)O(2)- and etoposide-induced apoptosis in HUVEC. Caspase-3 and caspase-9 activity was lower in Klotho-treated HUVEC than in control cells. Senescence-associated beta-gal staining showed that Klotho protein interferes with H(2)O(2)-induced premature cellular senescence. The expression of p53 and p21 was lower in Klotho-treated cells. Our study suggests that Klotho acts as a humoral factor to reduce H(2)O(2)-induced apoptosis and cellular senescence in vascular cells.
Mesh Headings (Keywords): Apoptosis, Cell Aging, Cells, Cultured, Endothelial Cells, Glucuronidase, Humans, Hydrogen Peroxide, Membrane Proteins, Oxidative Stress, Recombinant Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16325773
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