Recapitulation of Human Betab1-crystallin Promoter Activity in Transgenic Zebrafish.
From: Graduate Institute of Oral Biology, College of Medicine, National Taiwan University, Taipei, Taiwan, Republic of China.
Developmental dynamics : an official publication of the American Association of Anatomists
- Publish Date: Feb 2006
- ISSN: 1058-8388
- Volume: 235
- Issue: 2
- Pages: 435-43
- Medium: Print
- Language: English
- Citation (JAMA): Hou Hsin-Han, Kuo M Yen-Ping, Luo Ya-Wen, et al. Recapitulation of Human Betab1-crystallin Promoter Activity in Transgenic Zebrafish.. Dev. Dyn. Feb 2006;235:435-43
Abstract
Development of the eye is morphologically similar among vertebrates, indicating that the underlying mechanism regulating the process may have been highly conserved during evolution. Herein we analyzed the promoter of the human betaB1-crytallin gene in zebrafish by transgenic experiments. To delineate the evolutionarily conserved regulatory elements, we performed serial deletion assays in the promoter region. The results demonstrated that the -90/+61-bp upstream proximal promoter region is sufficient to confer lens-tissue specificity to the human betaB1-crystallin gene in transgenic zebrafish. Through phylogenetic sequence comparisons and an electrophoretic mobility shift assay (EMSA), a highly conserved cis-element of a six-base pair sequence TG(A/C)TGA, the consensus sequence for the Maf protein binding site, within the proximal promoter region was revealed. Further, a site-mutational assay showed that this element is crucial for promoter activity. These data suggest that the fundamental transcriptional regulatory mechanism of the betaB1-crystallin gene has been well conserved between humans and zebrafish, and plausibly among all vertebrates, during evolution.
Mesh Headings (Keywords): Animals, Animals, Genetically Modified, Base Sequence, Binding Sites, Conserved Sequence, Humans, Molecular Sequence Data, Phylogeny, Promoter Regions (Genetics), Proto-Oncogene Proteins c-maf, Sequence Alignment, Sequence Homology, Nucleic Acid, Transcription, Genetic, Zebrafish, beta-Crystallin B Chain
Check for Full Text / PubMed Unique Identifier (PMID): 16331646
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