Immune Suppressor Factor Confers Stromal Cell Line with Enhanced Supporting Activity for Hematopoietic Stem Cells.
From: Center of Excellence, The Institute of Medical Science, The University of Tokyo, 4-6-1 Shirokanedai, Minato-ku, Tokyo 108-8639, Japan. hnakajim@ims.u-tokyo.ac.jp
Biochemical and biophysical research communications
- Publish Date: Feb 2006
- ISSN: 0006-291X
- Volume: 340
- Issue: 1
- Pages: 35-42
- Medium: Print
- Language: English
- Citation (JAMA): Nakajima Hideaki, Shibata Fumi, Fukuchi Yumi, et al. Immune Suppressor Factor Confers Stromal Cell Line with Enhanced Supporting Activity for Hematopoietic Stem Cells.. Biochem. Biophys. Res. Commun. Feb 2006;340:35-42
Abstract
Immune suppressor factor (ISF) is a subunit of the vacuolar ATPase proton pump. We earlier identified a short form of ISF (ShIF) as a stroma-derived factor that supports cytokine-independent growth of mutant Ba/F3 cells. Here, we report that ISF/ShIF supports self-renewal and expansion of primary hematopoietic stem cells (HSCs). Co-culture of murine bone marrow cells with a stromal cell line overexpressing ISF or ShIF (MS10/ISF or MS10/ShIF) not only enhanced their colony-forming activity and the numbers of long-term culture initiating cells, but also maintained the competitive repopulating activity of HSC. This stem cell supporting activity depended on the proton-transfer function of ISF/ShIF. Gene expression analysis of ISF/ShIF-transfected cell lines revealed down-regulation of secreted frizzled-related protein-1 and tissue inhibitor of metalloproteinase-3, and the restoration of their expressions in MS10/ISF cells partially reversed its enhanced LTC-IC supporting activity to a normal level. These results suggest that ISF/ShIF confers stromal cells with enhanced supporting activities for HSCs by modulating Wnt-activity and the extracellular matrix.
Mesh Headings (Keywords): Animals, Cell Communication, Cell Culture Techniques, Cell Differentiation, Cell Proliferation, Cells, Cultured, Coculture Techniques, Hematopoietic Stem Cells, Mice, Mice, Inbred C57BL, Proton Pumps, Stromal Cells
Check for Full Text / PubMed Unique Identifier (PMID): 16343424
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.