Anucleate Cell Blue Assay: a Useful Tool for Identifying Novel Type Ii Topoisomerase Inhibitors.
From: Pharmacology & Microbiology Research Laboratories, Dainippon Pharmaceutical Co., Ltd., Enoki 33-94, Suita, Osaka 564-0053, Japan.
Antimicrobial agents and chemotherapy
- Publish Date: Jan 2006
- ISSN: 0066-4804
- Volume: 50
- Issue: 1
- Pages: 348-50
- Medium: Print
- Language: English
- Citation (JAMA): Oyamada Yoshihiro, Ito Hideaki, Fujimoto-Nakamura Mika, et al. Anucleate Cell Blue Assay: a Useful Tool for Identifying Novel Type Ii Topoisomerase Inhibitors.. Antimicrob. Agents Chemother. Jan 2006;50:348-50
Abstract
About 95,000 compounds were screened by the anucleate cell blue assay. Fifty-one of the hit compounds had various structures and showed inhibitory activity against DNA gyrase and/or topoisomerase IV. Moreover, the compounds exhibited antibacterial activity against a fluoroquinolone- and novobiocin-resistant strain of Staphylococcus aureus. The anucleate cell blue assay is therefore a useful tool for finding novel type II topoisomerase inhibitors.
Mesh Headings (Keywords): Bacteria, Biological Assay, Coloring Agents, DNA Topoisomerases, Type II, Enzyme Inhibitors, Fluoroquinolones
Check for Full Text / PubMed Unique Identifier (PMID): 16377708
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