Nucleocytoplasmic Shuttling of Bovine Herpesvirus 1 Ul47 Protein in Infected Cells.
From: Virus Assembly Group, Marie Curie Research Institute, Oxted, Surrey RH8 OTL, United Kingdom.
Journal of virology
- Publish Date: Jan 2006
- ISSN: 0022-538X
- Volume: 80
- Issue: 2
- Pages: 1059-63
- Medium: Print
- Language: English
- Citation (JAMA): Verhagen Janneke, Hutchinson Ian, Elliott Gillian, et al. Nucleocytoplasmic Shuttling of Bovine Herpesvirus 1 Ul47 Protein in Infected Cells.. J. Virol. Jan 2006;80:1059-63
Abstract
Previous studies with transfected cells have shown that the herpes simplex virus type 1 (HSV-1) and bovine herpesvirus 1 (BHV-1) UL47 proteins shuttle between the nucleus and the cytoplasm. HSV-1 UL47 has also been shown to bind RNA. Here we examine the BHV-1 UL47 protein in infected cells using a green fluorescent protein-UL47-expressing virus. We show that UL47 is detected in the nucleus early in infection. We use fluorescence loss in photobleaching to show that nuclear UL47 undergoes rapid nucleocytoplasmic shuttling. Furthermore, we demonstrate that actinomycin D inhibits the reaccumulation of UL47 in the nuclei of infected cells. These results suggest that UL47 exhibits behavior similar to that of previously characterized RNA-transporting proteins.
Mesh Headings (Keywords): Animals, Cell Line, Cell Nucleus, Cytoplasm, Herpesviridae Infections, Herpesvirus 1, Bovine, Viral Envelope Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16379009
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