Loss of Primary Cilia Results in Deregulated and Unabated Apical Calcium Entry in Arpkd Collecting Duct Cells.
From: Department of Physiology, Univ. of Alabama at Birmingham, Birmingham, AL, USA.
American journal of physiology. Renal physiology
- Publish Date: Jun 2006
- ISSN: 0363-6127
- Volume: 290
- Issue: 6
- Pages: F1320-8
- Medium: Print
- Language: English
- Citation (JAMA): Siroky Brian J, Ferguson William B, Fuson Amanda L, et al. Loss of Primary Cilia Results in Deregulated and Unabated Apical Calcium Entry in Arpkd Collecting Duct Cells.. Am. J. Physiol. Renal Physiol. Jun 2006;290:F1320-8
Abstract
Recent genetic analysis has identified a pivotal role of primary cilia in the pathogenesis of polycystic kidney disease (PKD). However, little is known regarding how cilia loss/dysfunction contributes to cyst development. In epithelial cells, changes in apical fluid flow induce cilia-mediated Ca2+ entry via polycystin-2 (PC2), a cation channel. The Oak Ridge Polycystic Kidney (orpk) mouse contains a mutated Tg737 gene that disrupts expression of polaris, a protein required for ciliogenesis. These studies examine the effect of cilia malformation on Ca2+ entry in orpk cilia(-) collecting duct principal cells, and in orpk cells in which wild-type Tg737 was reintroduced, orpk cilia(+). [Ca2+]i was monitored in confluent cell monolayers using fluorescence microscopy. Intrinsic apical Ca2+ entry was measured by Mn2+ quenching and Ca2+ depletion/readdition under flow conditions below the threshold for stimulation. We found that unstimulated apical Ca2+ entry was markedly increased in cilia(-) cells and was sensitive to Gd3+, an inhibitor of PC2. Electrophysiological measurements demonstrate increased abundance of an apical channel, consistent with PC2, in cilia(-) cells. Immunofluorescence studies revealed that PC2, normally expressed on and at the base of cilia in orpk cilia(+) cells, was observed throughout the apical membrane in cilia(-) cells. Furthermore, cilia(-) cells displayed elevated subapical Ca2+ levels measured with the near-membrane Ca2+ indicator FFP-18. We propose that cilia exert a tonic regulatory influence on apical Ca2+ entry, and absence of cilia results in loss of spatial organization of PC2, causing unregulated Ca2+ entry and elevations in subapical [Ca2+], a factor which may contribute to cyst formation.
Mesh Headings (Keywords): Animals, Calcium, Cell Membrane Permeability, Cells, Cultured, Cilia, Fluorescent Antibody Technique, Fluorescent Dyes, Fura-2, Gadolinium, Kidney Tubules, Collecting, Manganese, Mice, Polycystic Kidney, Autosomal Dominant, TRPP Cation Channels, Tumor Suppressor Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16396941
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