Targeted Deletion of Puma Attenuates Cardiomyocyte Death and Improves Cardiac Function During Ischemia-reperfusion.
From: Boston Biomedical Research Institute, Watertown, MA 02472, USA.
American journal of physiology. Heart and circulatory physiology
- Publish Date: Jul 2006
- ISSN: 0363-6135
- Volume: 291
- Issue: 1
- Pages: H52-60
- Medium: Print
- Language: English
- Citation (JAMA): Toth Ambrus, Jeffers John R, Nickson Philip, et al. Targeted Deletion of Puma Attenuates Cardiomyocyte Death and Improves Cardiac Function During Ischemia-reperfusion.. Am. J. Physiol. Heart Circ. Physiol. Jul 2006;291:H52-60
Abstract
The p53-upregulated modulator of apoptosis (Puma), a BH3-only member of the Bcl-2 protein family, is required for p53-dependent and -independent forms of apoptosis and has been implicated in the pathomechanism of several diseases, including cancer, acquired immunodeficiency syndrome, and ischemic brain disease. The role of Puma in cardiomyocyte death, however, has not been analyzed. On the basis of the ability of Puma to integrate diverse cell death stimuli, we hypothesized that Puma might be critical for cardiomyocyte death upon ischemia-reperfusion (I/R) of the heart. Here we show that hypoxia-reoxygenation of isolated cardiomyocytes led to an increase in Puma mRNA and protein levels. Moreover, if Puma was delivered by an adenoviral construct, cardiomyocytes died by apoptosis. Under ATP-depleted conditions, however, Puma overexpression primarily induced necrosis, suggesting that Puma is involved in the development of both types of cell death. Consistent with these findings, targeted deletion of Puma in a mouse model attenuated both apoptosis and necrosis. When the Langendorff ex vivo I/R model was used, infarcts were approximately 50% smaller in Puma(-/-) than in wild-type mice. As a result, after I/R, cardiac function was significantly better preserved in Puma(-/-) mice than in their wild-type littermates. Our study thus establishes Puma as an essential mediator of cardiomyocyte death upon I/R injury and offers a novel therapeutic target to limit cell loss in ischemic heart disease.
Mesh Headings (Keywords): Animals, Animals, Newborn, Apoptosis, Apoptosis Regulatory Proteins, Cells, Cultured, Drug Delivery Systems, Gene Deletion, Gene Targeting, Myocytes, Cardiac, Proto-Oncogene Proteins, Rats, Rats, Sprague-Dawley, Reperfusion Injury, Treatment Outcome, Ventricular Dysfunction, Left
Check for Full Text / PubMed Unique Identifier (PMID): 16399862
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.