Reactive Nitrogen Species Augment Fibroblast-mediated Collagen Gel Contraction, Mediator Production, and Chemotaxis.
From: University of Nebraska Medical Center, 985885 Nebraska Medical Center, Omaha, NE 68198-5885, USA. srennard@unmc.edu
American journal of respiratory cell and molecular biology
- Publish Date: May 2006
- ISSN: 1044-1549
- Volume: 34
- Issue: 5
- Pages: 592-9
- Medium: Print
- Language: English
- Citation (JAMA): Sugiura Hisatoshi, Liu Xiangde, Kobayashi Tetsu, et al. Reactive Nitrogen Species Augment Fibroblast-mediated Collagen Gel Contraction, Mediator Production, and Chemotaxis.. Am. J. Respir. Cell Mol. Biol. May 2006;34:592-9
Abstract
Reactive nitrogen species (RNS) such as peroxynitrite cause cellular injury and tissue inflammation. Excessive production of nitrotyrosine, which is a footprint of RNS, has been observed in the airways of patients with asthma and chronic obstructive pulmonary disease, disorders characterized by tissue remodeling. The aim of this study was to evaluate whether RNS can affect tissue remodeling through direct effects on fibroblasts, and to determine if these effects depend on production of transforming growth factor-beta (TGF-beta). To accomplish this, human fetal lung fibroblasts (HFL-1) were used to assess fibroblast-mediated contraction of floating gels and chemotaxis toward fibronectin. In addition, the ability of fibroblasts to release TGF-beta1, fibronectin, and vascular endothelial growth factor (VEGF) was assessed by enzyme-linked immunosorbent assay. Authentic peroxynitrite significantly augmented gel contraction (P < 0.01) and chemotaxis (P < 0.01) compared with control in a concentration-dependent manner. Similarly, the peroxynitrite donor 3-morpholynosidenonimine hydrochloride (SIN-1) also augmented gel contraction (P < 0.01). RNS also significantly increased TGF-beta1 (P < 0.01), fibronectin (P < 0.01), and VEGF (P < 0.01) release into the media in both 3D gel and monolayer culture. Anti-TGF-beta antibody reversed RNS-augmented gel contraction (P < 0.01) and mediator production (P < 0.01). Anti-TGF-beta antibody also partially, but significantly, reversed RNS-augmented chemotaxis toward fibronectin (P < 0.01). Finally, peroxynitrite enhanced expression of alpha5beta1 integrin, which is a receptor for fibronectin (P < 0.01), and neutralizing anti-TGF-beta antibody suppressed peroxynitrite-augmented alpha5beta1 expression (P < 0.01). These results suggest that RNS can affect the tissue repair process by modulating TGF-beta1.
Mesh Headings (Keywords): Animals, Antibodies, Cells, Cultured, Chemotaxis, Collagen, Fibroblasts, Fibronectins, Gels, Humans, Hydrogen Peroxide, Integrin alpha5beta1, Peroxynitrous Acid, Rats, Reactive Nitrogen Species, Time Factors, Transforming Growth Factor beta, Transforming Growth Factor beta1, Vascular Endothelial Growth Factor A
Check for Full Text / PubMed Unique Identifier (PMID): 16399954
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