Vascular Endothelial Growth Factor Signalling in Endothelial Cell Survival: a Role for Nfkappab.
From: Kennedy Institute of Rheumatology, Faculty of Medicine, Imperial College, London, UK. Jennifer.grosjean@imperial.ac.uk
Biochemical and biophysical research communications
- Publish Date: Feb 2006
- ISSN: 0006-291X
- Volume: 340
- Issue: 3
- Pages: 984-94
- Medium: Print
- Language: English
- Citation (JAMA): Grosjean Jennifer, Kiriakidis Serafim, Reilly Kerri, et al. Vascular Endothelial Growth Factor Signalling in Endothelial Cell Survival: a Role for Nfkappab.. Biochem. Biophys. Res. Commun. Feb 2006;340:984-94
Abstract
Angiogenesis is the development of blood capillaries from pre-existing vessels. Vascular endothelial growth factor (VEGF) is a key regulator of vessel growth and regression, and acts as an endothelial survival factor by protecting endothelial cells from apoptosis. Many genes involved in cell proliferation and apoptosis are regulated by the nuclear factor kappa B (NFkappaB) transcription factor family. This study aimed to address the hypothesis that VEGF-mediated survival effects on endothelium involve NFkappaB. Using an NFkappaB-luciferase reporter adenovirus, we observed activation of NFkappaB following VEGF treatment of human umbilical vein endothelial cells. This was confirmed using electrophoretic mobility shift assay and found to involve nuclear translocation of NFkappaB sub-unit p65. However, NFkappaB activation occurred without degradation of inhibitory IkappaB proteins (IkappaBalpha, IkappaBbeta, and IkappaBepsilon). Instead, tyrosine phosphorylation of IkappaBalpha was observed following VEGF treatment, suggesting NFkappaB activation was mediated by degradation-independent dissociation of IkappaBalpha from NFkappaB. Adenovirus-mediated over-expression of either native IkappaBalpha, or of IkappaBalpha in which tyrosine residue 42 was mutated to phenylalanine, inhibited induction of NFkappaB-dependent luciferase activity in response to VEGF. Furthermore, VEGF-induced upregulation of mRNA for the anti-apoptotic protein Bcl-2 and cell survival following serum withdrawal was reduced following IkappaBalpha over-expression. This study highlights that different molecular mechanisms of NFkappaB activation may be involved downstream of stimuli which activate the endothelial lining of blood vessels.
Mesh Headings (Keywords): Adenoviridae, Apoptosis, Blotting, Western, Cell Survival, Cells, Cultured, Endothelial Cells, Endothelium, Vascular, Humans, Luciferases, NF-kappa B, Neovascularization, Pathologic, Phosphorylation, RNA, Messenger, Signal Transduction, Time Factors, Transcription Factor RelA, Transcription Factors, Transcription, Genetic, Tyrosine, Umbilical Veins, Up-Regulation, Vascular Endothelial Growth Factor A
Check for Full Text / PubMed Unique Identifier (PMID): 16410078
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.