Early Induction of Calpains in Rotenone-mediated Neuronal Apoptosis.
From: Department of Biochemistry, Yong Loo Lin School of Medicine, National University of Singapore, 8 Medical Drive, Singapore 117597, Republic of Singapore.
Neuroscience letters
- Publish Date:
- ISSN: 0304-3940
- Volume: 397
- Issue: 1-2
- Pages: 69-73
- Medium: Print
- Language: English
- Citation (JAMA): Chen Minghui Jessica, Yap Yann Wan, Choy Meng Shyan, et al. Early Induction of Calpains in Rotenone-mediated Neuronal Apoptosis.. Neurosci. Lett. ;397:69-73
Abstract
Rotenone is an inhibitor of mitochondrial complex I that produces a model of Parkinson’s disease (PD), where neurons undergo apoptosis by caspase-dependent and/or caspase-independent pathways. Inhibition of calpains has recently been shown to attenuate neuronal apoptosis. This study aims to establish for the first time, the time-point of calpain activation with respect to the caspase activation and the possibility of cell cycle re-entry in rotenone-mediated cell death. Immunoblot results revealed calpain activation occurred at 5, 10h prior to caspase-3 activation (at 15 h), suggesting calpain activation was an earlier cellular event compared to caspase activation in the rotenone-mediated apoptosis. In addition, an upregulation of phospho-p53 was observed at 21 h. However, no expression or upregulation of cell cycle regulatory proteins including cdc25a, cyclin-D1 and cyclin-D3 were observed, strongly suggesting that cell cycle re-entry did not occur. These findings provide new insights into the differential patterns of calpain and caspase activation that result from rotenone poisoning and which may be relevant to the therapeutic management of PD.
Mesh Headings (Keywords): Analysis of Variance, Animals, Apoptosis, Blotting, Western, Calpain, Carrier Proteins, Caspase 3, Caspases, Cell Survival, Cells, Cultured, Cerebral Cortex, Dose-Response Relationship, Drug, Embryo, Mammalian, Enzyme Induction, Gene Expression Regulation, Insecticides, Mice, Microfilament Proteins, Neurons, Rats, Rotenone, Time Factors, Tumor Suppressor Protein p53
Check for Full Text / PubMed Unique Identifier (PMID): 16412576
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.