Transduction of Nondividing Human Macrophages with Gammaretrovirus-derived Vectors.
From: LaboRetro, INSERM U412, Ecole Normale Supérieure de Lyon, IFR 128 BioSciences Lyon-Gerland, 46 Allée d’Italie, 69364 Lyon, France.
Journal of virology
- Publish Date: Feb 2006
- ISSN: 0022-538X
- Volume: 80
- Issue: 3
- Pages: 1152-9
- Medium: Print
- Language: English
- Citation (JAMA): Jarrosson-Wuilleme Loraine, Goujon Caroline, Bernaud Jeanine, et al. Transduction of Nondividing Human Macrophages with Gammaretrovirus-derived Vectors.. J. Virol. Feb 2006;80:1152-9
Abstract
It is commonly accepted that infection of nondividing cells by gammaretroviruses such as the murine leukemia viruses is inefficient due to their inability to cross the nuclear envelope barrier. Challenging this notion, we now show that human nondividing macrophages display a specific window of susceptibility to transduction with a Friend murine leukemia virus (F-MLV)-derived vector during their differentiation from monocytes. This finding suggests that factors other than the nuclear membrane govern permissiveness to gammaretroviral infection and raises the possibility of using the macrophage tropism of F-MLV in gene therapy.
Mesh Headings (Keywords): Animals, Base Sequence, Cell Differentiation, Cell Proliferation, DNA, Viral, Friend murine leukemia virus, Gene Therapy, Genetic Vectors, Granulocyte Macrophage Colony-Stimulating Factors, Recombinant, Hela Cells, Humans, Macrophages, Mice, Moloney murine leukemia virus, Nuclear Envelope, Transduction, Genetic
Check for Full Text / PubMed Unique Identifier (PMID): 16414992
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