Genetic Manipulation of an Exogenous Non-immunoglobulin Protein by Gene Conversion Machinery in a Chicken B Cell Line.
From: Department of Biotechnology, Okayama University, Tsushima-Naka, 3-1-1, Okayama 700-8530, Japan. nkanayam@cc.okayama-u.ac.jp
Nucleic acids research
- Publish Date: 2006
- ISSN: 1362-4962
- Volume: 34
- Issue: 2
- Pages: e10
- Medium: Internet
- Language: English
- Citation (JAMA): Kanayama Naoki, Todo Kagefumi, Takahashi Satoko, et al. Genetic Manipulation of an Exogenous Non-immunoglobulin Protein by Gene Conversion Machinery in a Chicken B Cell Line.. Nucleic Acids Res. 2006;34:e10
Abstract
During culture, a chicken B cell line DT40 spontaneously mutates immunoglobulin (Ig) genes by gene conversion, which involves activation-induced cytidine deaminase (AID)-dependent homologous recombination of the variable (V) region gene with upstream pseudo-V genes. To explore whether this mutation mechanism can target exogenous non-Ig genes, we generated DT40 lines that bears a gene conversion substrate comprising the green fluorescent protein (GFP) gene as a donor and the blue fluorescent protein (BFP) gene as an acceptor. A few percent of the initially BFP-expressing cells converted their fluorescence from blue to green after culture for 2-3 weeks when the substrate construct was integrated in the Ig light chain locus, but not in the ovalbumin locus. This was the result of AID-dependent and the GFP gene-templated gene conversion of the BFP gene, thereby leading to the introduction of various sizes of GFP-derived gene segment into the BFP gene. Thus, G/B construct may be used to visualize gene conversion events. After switching off AID expression in DT40 cells, the mutant clones were isolated stably and maintained with their mutations being fixed. Thus, the gene conversion machinery in DT40 cells will be a useful means to engineer non-Ig proteins by a type of DNA shuffling.
Mesh Headings (Keywords): Animals, B-Lymphocytes, Cell Line, Chickens, Cytidine Deaminase, DNA Shuffling, Gene Conversion, Genes, Immunoglobulin Light Chain, Green Fluorescent Proteins, Luminescent Agents, Luminescent Proteins
Check for Full Text / PubMed Unique Identifier (PMID): 16421270
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