Opioids Trigger Alpha 5 Beta 1 Integrin-mediated Monocyte Adhesion.
From: Department of Immunology and Oncology, Centro Nacional de Biotecnología/Consejo Superior de Investigaciones Científicas, Madrid, Spain.
Journal of immunology (Baltimore, Md. : 1950)
- Publish Date: Feb 2006
- ISSN: 0022-1767
- Volume: 176
- Issue: 3
- Pages: 1675-85
- Medium: Print
- Language: English
- Citation (JAMA): Pello Oscar M, Duthey Béatrice, García-Bernal David, et al. Opioids Trigger Alpha 5 Beta 1 Integrin-mediated Monocyte Adhesion.. J. Immunol. Feb 2006;176:1675-85
Abstract
Inflammatory reactions involve a network of chemical and molecular signals that initiate and maintain host response. In inflamed tissue, immune system cells generate opioid peptides that contribute to potent analgesia by acting on specific peripheral sensory neurons. In this study, we show that opioids also modulate immune cell function in vitro and in vivo. By binding to its specific receptor, the opioid receptor-specific ligand DPDPE triggers monocyte adhesion. Integrins have a key role in this process, as adhesion is abrogated in cells treated with specific neutralizing anti-alpha5beta1 integrin mAb. We found that DPDPE-triggered monocyte adhesion requires PI3Kgamma activation and involves Src kinases, the guanine nucleotide exchange factor Vav-1, and the small GTPase Rac1. DPDPE also induces adhesion of pertussis toxin-treated cells, indicating involvement of G proteins other than Gi. These data show that opioids have important implications in regulating leukocyte trafficking, adding a new function to their known effects as immune response modulators.
Mesh Headings (Keywords): Animals, Cell Adhesion, Cell Line, Cells, Cultured, Enkephalin, D-Penicillamine (2,5)-, Humans, Integrin alpha5beta1, Mice, Mice, Inbred BALB C, Monocytes, Narcotics, Proto-Oncogene Proteins c-vav, rac1 GTP-Binding Protein, src-Family Kinases
Check for Full Text / PubMed Unique Identifier (PMID): 16424197
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.