Dendritic Cells Transduced with Recombinant Adenoviruses Induce More Efficient Anti-tumor Immunity Than Dendritic Cells Pulsed with Peptide.
From: Department of Surgery, Kang-Nam St. Mary’s Hospital, Catholic University of Korea, 505 Banpo-Dong, Seocho-Gu, Seoul 137-701, South Korea.
Vaccine
- Publish Date: Apr 2006
- ISSN: 0264-410X
- Volume: 24
- Issue: 15
- Pages: 2860-8
- Medium: Print
- Language: English
- Citation (JAMA): Oh Seong-Taek, Kim Chang-Hyun, Park Mi-Young, et al. Dendritic Cells Transduced with Recombinant Adenoviruses Induce More Efficient Anti-tumor Immunity Than Dendritic Cells Pulsed with Peptide.. Vaccine Apr 2006;24:2860-8
Abstract
Transduction with recombinant, replication-defective adenovirus (AdV) vectors encoding a transgene is an efficient method for gene transfer into murine or human dendritic cells (DC). We previously reported that human dendritic cells transduced with recombinant adenovirus encoding the CEA gene (AdVCEA) can effectively induce antigen-specific cytotoxic T lymphocytes (CTL) in vitro. In this study, the efficacy of vaccination using AdVCEA-transduced DC was compared with peptide-pulsed DC in terms of the antigen-specific CTL activity and anti-tumor immunity to MC38/CEA2 in a murine tumor model. AdVCEA-transduced DC increased antigen-specific T-cell proliferation, augmented the number of IFN-gamma secreting T-cells and induced potent CEA-specific CTL capable of lysing target cells pulsed with CEA peptide, as well as MC38/CEA2 expressing CEA, compared to peptide-pulsed DC. Moreover, vaccination of mice with AdVCEA-transduced DC induced a potent protective and therapeutic anti-tumor immunity to MC38/CEA2 in a subcutaneous model. These data suggest that AdVCEA-transduced DC appears to be superior to peptide-pulsed DC for the induction of anti-tumor immunity against tumor cells; this occurs through augmentation of the antigen-specific CTL response and may be used as an efficient DC-based tumor vaccine applicable to clinical care.
Mesh Headings (Keywords): Adenoviridae, Animals, Cancer Vaccines, Carcinoembryonic Antigen, Cells, Cultured, Cytotoxicity, Immunologic, Dendritic Cells, Female, Interferon Type II, Mice, Mice, Inbred C57BL, Neoplasms, T-Lymphocytes, Cytotoxic, Transduction, Genetic, Vaccines, Subunit, Vaccines, Synthetic
Check for Full Text / PubMed Unique Identifier (PMID): 16443309
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.