Identification of Three Novel Ostricacins: an Update on the Phylogenetic Perspective of Beta-defensins.
From: Biotechnology Group, Institute of Technology and Engineering, Massey University, Private Bag 11-222, Palmerston North 5301, New Zealand.
International journal of antimicrobial agents
- Publish Date: Mar 2006
- ISSN: 0924-8579
- Volume: 27
- Issue: 3
- Pages: 229-35
- Medium: Print
- Language: English
- Citation (JAMA): Sugiarto Haryadi, Yu Pak-Lam, et al. Identification of Three Novel Ostricacins: an Update on the Phylogenetic Perspective of Beta-defensins.. Int. J. Antimicrob. Agents Mar 2006;27:229-35
Abstract
Three new beta-defensins, ostricacins-2, 3 and 4 (Osp-2, 3 and 4), have been successfully purified and characterised from ostrich heterophils in addition to ostricacin-1 (Osp-1). These peptides are composed of 36-42 amino acids with a molecular weight range of 4.70-4.98 kDa. In vitro, Osp-1, 3 and 4 were active against Escherichia coli O157:H7 and Staphylococcus aureus 1056 MRSA, whilst Osp-2 was active against bacterial strains plus the yeast Candida albicans 3153A. Minimal inhibitory concentrations of the three ostricacins ranged from 0.96 microg/mL to 12.03 microg/mL. Comparison with the known beta-defensins from mammalian and other avian species revealed that the four ostricacins shared eight conserved residues (six cysteines and two glycines), identified as the ‘beta-defensin core motif’. Comparisons of the sequence also indicated that beta-defensins could have originated from a common beta-defensin-like ancestor that occurred before avian and mammalian lines diverged.
Mesh Headings (Keywords): Animals, Bacteria, Microbial Sensitivity Tests, Phylogeny, Struthioniformes, beta-Defensins
Check for Full Text / PubMed Unique Identifier (PMID): 16459058
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