Efficient Inhibition of Hiv-1 Replication in Human Immature Monocyte-derived Dendritic Cells by Purified Anti-hiv-1 Igg Without Induction of Maturation.
From: Institut de Virologie, 3 rue Koeberlé, F-67000 Strasbourg, France. vincent.holl@hemato-ulp.u-strasbg.fr
Blood
- Publish Date: Jun 2006
- ISSN: 0006-4971
- Volume: 107
- Issue: 11
- Pages: 4466-74
- Medium: Print
- Language: English
- Citation (JAMA): Holl Vincent, Peressin Maryse, Schmidt Sylvie, et al. Efficient Inhibition of Hiv-1 Replication in Human Immature Monocyte-derived Dendritic Cells by Purified Anti-hiv-1 Igg Without Induction of Maturation.. Blood Jun 2006;107:4466-74
Abstract
During mucosal HIV transmission, immature dendritic cells (DCs) present in the mucosa are among the first cellular targets of the virus. Previous studies have analyzed the inhibition of HIV-1 transfer from human mature DCs to T lymphocytes by neutralizing IgG, but so far no in vitro data regarding the capacity of antibodies to inhibit HIV-1 infection of immature DCs have been reported. Here, we found an increased HIV-inhibitory activity of monoclonal IgG and purified polyclonal IgG when immature monocyte-derived dendritic cells (iMDDCs) were used as target cells instead of autologous blood lymphocytes. We showed that FcgammaRII is involved in the mechanism for inhibiting HIV-1 infection of iMDDCs by IgG, whereas no induction of maturation was detected at concentrations of IgG that result in a 90% reduction of HIV replication. After induction of FcgammaRI expression on iMDDCs by IFN-gamma, an augmentation of the HIV-inhibitory activity of IgG, related to the expression of FcgammaRI, was observed. Taken together, our results demonstrate the participation of FcgammaRs in HIV-1 inhibition by IgG when iMDDCs are the targets. We propose that IgG is able to efficiently inhibit HIV-1 replication in iMDDCs and should be one of the components to be induced by vaccination.
Mesh Headings (Keywords): Cell Differentiation, Cells, Cultured, Dendritic Cells, Gene Expression Regulation, HIV Antibodies, HIV Infections, HIV-1, Humans, Immunoglobulin G, Interferon Type II, Receptors, IgG, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 16469871
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