Dual Mutations in the Autographa Californica Nucleopolyhedrovirus Fp-25 and P35 Genes Result in Plasma-membrane Blebbing in Trichoplusia Ni Cells.
From: Centre for Ecology and Hydrology Oxford, Mansfield Road, Oxford OX1 3SR, UK.
The Journal of general virology
- Publish Date: Mar 2006
- ISSN: 0022-1317
- Volume: 87
- Issue: Pt 3
- Pages: 531-6
- Medium: Print
- Language: English
- Citation (JAMA): Kelly Barbara J, King Linda A, Possee Robert D, et al. Dual Mutations in the Autographa Californica Nucleopolyhedrovirus Fp-25 and P35 Genes Result in Plasma-membrane Blebbing in Trichoplusia Ni Cells.. J. Gen. Virol. Mar 2006;87:531-6
Abstract
Spodoptera frugiperda cells infected with Autographa californica nucleopolyhedrovirus (AcMNPV) lacking a functional anti-apoptotic p35 protein undergo apoptosis. However, such mutants replicate normally in Trichoplusia ni (TN-368) cells. An AcMNPV plaque isolate (AcdefrT) was identified during propagation of a virus deficient in p35 in TN-368 cells. This virus exhibited enhanced budded-particle formation in TN-368 cells, but was partially defective for polyhedra production in the same cells. Virus replication in AcdefrT-infected TN-368 cells was accompanied by extensive plasma-membrane blebbing and caspase activation late in infection, both features of apoptosis. Rescue of the p35 locus of AcdefrT continued to result in a reduction in polyhedra and increase in budded virus production in TN-368 cells, but no plasma-membrane blebbing was observed. The mutation was mapped to the FP-25 gene locus. This gene mutation combined with the non-functional p35 was found to be responsible for the cell-blebbing effect observed in AcdefrT-infected TN-368 cells.
Mesh Headings (Keywords): Animals, Base Sequence, Caspase 3, Caspases, Cell Line, Cell Membrane, Genes, Viral, Molecular Sequence Data, Moths, Mutation, Nucleocapsid Proteins, Nucleopolyhedrovirus, Viral Proteins, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 16476974
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