Putative Neutralization Epitopes and Broad Cross-genotype Neutralization of Hepatitis E Virus Confirmed by a Quantitative Cell-culture Assay.
From: Molecular Hepatitis and Hepatitis Viruses Section, Laboratory of Infectious Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 50 South Drive MSC-8009, Bethesda, MD 20892-8009, USA. semerson@niaid.nih.gov
The Journal of general virology
- Publish Date: Mar 2006
- ISSN: 0022-1317
- Volume: 87
- Issue: Pt 3
- Pages: 697-704
- Medium: Print
- Language: English
- Citation (JAMA): Emerson Suzanne U, Clemente-Casares Pilar, Moiduddin Nasser, et al. Putative Neutralization Epitopes and Broad Cross-genotype Neutralization of Hepatitis E Virus Confirmed by a Quantitative Cell-culture Assay.. J. Gen. Virol. Mar 2006;87:697-704
Abstract
Monolayers of Hep G2/C3A cells were inoculated with genotype 1 Hepatitis E virus (HEV) mixed with either anti-HEV or an appropriate control. After 5 or 6 days, cell monolayers were stained with anti-HEV and infected cells were identified by immunofluorescence microscopy and counted. Anti-HEV from vaccinated or infected rhesus monkeys neutralized the virus, as did mAbs that recognized epitopes on the C terminus of a recombinant vaccine protein. Antibodies were broadly cross-reactive, since convalescent serum from animals infected with any one of the four mammalian genotypes all neutralized the genotype 1 virus.
Mesh Headings (Keywords): Animals, Cell Line, Cross Reactions, Epitopes, Hepatitis Antibodies, Hepatitis E, Hepatitis E virus, Humans, Immune Sera, Immunoglobulin Fab Fragments, Macaca mulatta, Neutralization Tests, Species Specificity, Vaccination, Vaccines, Synthetic, Viral Hepatitis Vaccines
Check for Full Text / PubMed Unique Identifier (PMID): 16476993
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