Trkb Receptors Modulation of Glutamate Release is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus.
From: Department of Zoology, Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Journal of neuroscience research
- Publish Date: Apr 2006
- ISSN: 0360-4012
- Volume: 83
- Issue: 5
- Pages: 832-44
- Medium: Print
- Language: English
- Citation (JAMA): Pereira Daniela B, Rebola Nelson, Rodrigues Ricardo J, et al. Trkb Receptors Modulation of Glutamate Release is Limited to a Subset of Nerve Terminals in the Adult Rat Hippocampus.. J. Neurosci. Res. Apr 2006;83:832-44
Abstract
Brain-derived neurotrophic factor (BDNF) modulates glutamatergic excitatory transmission in hippocampal primary cultures by acting at a presynaptic locus. Although it has been suggested that BDNF also modulates adult hippocampus glutamatergic transmission, this remains a matter of controversy. To clarify a putative role for this neurotrophin in the modulation of glutamate release we applied exogenous BDNF to isolated adult rat hippocampal nerve terminals. BDNF, at 100 ng/ml, potentiated by 25% the K(+)-evoked release of [(3)H]glutamate from hippocampal synaptosomes. The small effect of BDNF on [(3)H]glutamate release correlated with a modest increase in phospholipase Cgamma (PLCgamma) phosphorylation, and with the lack of effect of BDNF on extracellular-signal regulated kinase (ERK) and Akt phosphorylation. Immunocytochemistry studies demonstrated that only about one-third of glutamatergic synaptosomes were positive for TrkB immunoreactivity. Furthermore, biotinylation and subsynaptic fractionation studies showed that only one-fourth of total full-length TrkB was present at the plasma membrane, evenly distributed between the presynaptic active zone and the postsynaptic density. These results indicate that BDNF modulates synaptic transmission presynaptically in a small subset of hippocampal glutamatergic synapses that contain TrkB and that express the receptor on the plasma membrane.
Mesh Headings (Keywords): Animals, Brain-Derived Neurotrophic Factor, Cells, Cultured, Extracellular Signal-Regulated MAP Kinases, Glutamic Acid, Hippocampus, Immunoblotting, Immunohistochemistry, Male, Presynaptic Terminals, Proto-Oncogene Proteins c-akt, Rats, Rats, Wistar, Receptor, trkB, Synaptosomes
Check for Full Text / PubMed Unique Identifier (PMID): 16477614
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