Cooperative Action of Sox9, Snail2 and Pka Signaling in Early Neural Crest Development.
From: Center for Translational and Advanced Animal Research on Human Diseases, Division of Developmental Neuroscience, Graduate School of Medicine, Tohoku University, Sendai, Miyagi 980-8575, Japan.
Development (Cambridge, England)
- Publish Date: Apr 2006
- ISSN: 0950-1991
- Volume: 133
- Issue: 7
- Pages: 1323-33
- Medium: Print
- Language: English
- Citation (JAMA): Sakai Daisuke, Suzuki Takashi, Osumi Noriko, et al. Cooperative Action of Sox9, Snail2 and Pka Signaling in Early Neural Crest Development.. Development Apr 2006;133:1323-33
Abstract
In neural crest formation, transcription factors, such as group E Sox and Snail1/Snail2 (Slug) regulate subsequent epithelial-mesenchymal transition (EMT) and migration. In particular, Sox9 has a strong effect on neural crest formation, EMT and differentiation of crest-derived cartilages in the cranium. It remains unclear, however, how Sox9 functions in these events, and how Sox9 activity is regulated. In this study, our gain-of-function and loss-of-function experiments reveal that Sox9 is essential for BMP signal-mediated induction of Snail2 and subsequent EMT in avian neural crest. We also show that Snail2 activates the Snail2 promoter, although Snail family proteins have been known as a repressor. Consistently, Sox9 directly activates the Snail2 promoter in synergy with, and through a direct binding to, Snail2. Finally, functions of these transcription factors in neural crest cells are enhanced by PKA signaling.
Mesh Headings (Keywords): Animals, Cell Differentiation, Coturnix, Electrophoretic Mobility Shift Assay, Embryo, Nonmammalian, Embryonic Development, Epithelium, Fluorescent Antibody Technique, Indirect, Fluorescent Dyes, Gene Expression Regulation, Developmental, Genes, Reporter, Green Fluorescent Proteins, Immunohistochemistry, In Situ Hybridization, Luciferases, Mesoderm, Models, Biological, Neural Crest, Organ Culture Techniques, Phalloidine, Precipitin Tests, Promoter Regions (Genetics), Signal Transduction, Transcription Factors
Check for Full Text / PubMed Unique Identifier (PMID): 16510505
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