Decreased Calcium/Calmodulin-dependent Protein Kinase Ii and Protein Kinase C Activities Mediate Impairment of Hippocampal Long-term Potentiation in the Olfactory Bulbectomized Mice.
From: Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Tohoku University, Sendai, Japan. shigeki@mail.pharm.tohoku.ac.jp
Journal of neurochemistry
- Publish Date: Apr 2006
- ISSN: 0022-3042
- Volume: 97
- Issue: 1
- Pages: 22-9
- Medium: Print
- Language: English
- Citation (JAMA): Moriguchi Shigeki, Han Feng, Nakagawasai Osamu, et al. Decreased Calcium/Calmodulin-dependent Protein Kinase Ii and Protein Kinase C Activities Mediate Impairment of Hippocampal Long-term Potentiation in the Olfactory Bulbectomized Mice.. J. Neurochem. Apr 2006;97:22-9
Abstract
Olfactory bulbectomized (OBX) mice showed significant impairment of learning and memory-related behaviors 14 days after olfactory bulbectomy, as measured by passive avoidance and Y-maze tasks. We here observed a large impairment of hippocampal long-term potentiation (LTP) in the OBX mice. Concomitant with decreased acetylcholinesterase expression, protein kinase C (PKC)alpha autophosphorylation and NR1(Ser-896) phosphorylation significantly decreased in the hippocampal CA1 region of OBX mice. Both PKCalpha and NR1(Ser-896) phosphorylation significantly increased following LTP in the control mice, whereas increases were not observed in OBX mice. Like PKC activities, calcium/calmodulin-dependent protein kinase II (CaMKII) autophosphorylation significantly decreased in the hippocampal CA1 region of OBX mice as compared with that of control mice. In addition, increased CaMKII autophosphorylation following LTP was not observed in OBX mice. Finally, the impairment of CaMKII autophosphorylation was closely associated with reduced pGluR1(Ser-831) phosphorylation, without change in synapsin I (site 3) phosphorylation in the hippocampal CA1 region of OBX mice. Taken together, in OBX mice NMDA receptor hypofunction, possibly through decreased PKCalpha activity, underlies decreased CaMKII activity in the post-synaptic regions, thereby impairing LTP induction in the hippocampal CA1 region. Both decreased PKC and CaMKII activities with concomitant LTP impairment account for the learning disability observed in OBX mice.
Mesh Headings (Keywords): Acetylcholine, Acetylcholinesterase, Alzheimer Disease, Animals, Calcium-Calmodulin-Dependent Protein Kinase Type 2, Calcium-Calmodulin-Dependent Protein Kinases, Cholinergic Fibers, Denervation, Disease Models, Animal, Down-Regulation, Efferent Pathways, Hippocampus, Learning Disorders, Long-Term Potentiation, Male, Memory Disorders, Mice, Olfactory Bulb, Organ Culture Techniques, Phosphorylation, Protein Kinase C-alpha, Receptors, AMPA, Receptors, Metabotropic Glutamate, Receptors, N-Methyl-D-Aspartate, Signal Transduction
Check for Full Text / PubMed Unique Identifier (PMID): 16515554
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