Prednisolone Inhibits Hyperosmolarity-induced Expression of Mcp-1 Via Nf-kappab in Peritoneal Mesothelial Cells.
From: The Second Department of Internal Medicine, University of Occupational and Environmental Health School of Medicine, and Kidney Center, University Hospital, Kitakyushu, Japan.
Kidney international
- Publish Date: Feb 2006
- ISSN: 0085-2538
- Volume: 69
- Issue: 4
- Pages: 736-46
- Medium: Print
- Language: English
- Citation (JAMA): Matsuo H, Tamura M, Kabashima N, et al. Prednisolone Inhibits Hyperosmolarity-induced Expression of Mcp-1 Via Nf-kappab in Peritoneal Mesothelial Cells.. Kidney Int. Feb 2006;69:736-46
Abstract
The mechanism of peritoneal fibrosis in patients on continuous ambulatory peritoneal dialysis (CAPD) is poorly elucidated. We investigated the cellular mechanism of high-glucose-induced expression of monocyte chemoattractant protein-1 (MCP-1), which is important in recruiting monocytes into the peritoneum and progression of peritoneal fibrosis, and examined the inhibitory mechanism of glucocorticoids. Rat peritoneal mesothelial cells were cultured in high-glucose-containing medium and then analyzed for phosphorylation levels of p42/44 and p38 mitogen-activated protein (MAP) kinases (MAPK), MAPK or extracellular signal-regulated kinase kinase (MEK)1/2, c-Jun N-terminal kinase (JNK)1/2, and protein kinase C (PKC) by Western blotting. Expression of MCP-1 was examined by reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay, respectively. DNA-binding activity of nuclear factor (NF)-kappaB was measured by electrophoretic mobility shift assay. High glucose increased MCP-1 mRNA and MCP-1 protein expression. Although glucose increased phosphorylation of MEK1/2, p42/44 MAPK, p38 MAPK, JNK1/2, and PKC, and DNA-binding activity of NF-kappaB, its effect on MCP-1 expression was suppressed only by PKC and NF-kappaB inhibitors. Mannitol caused a similar increase in PKC and NF-kappaB activation and MCP-1 synthesis. Prednisolone increased I-kappaB-alpha expression and inhibited glucose/mannitol-induced NF-kappaB DNA binding and MCP-1 expression without affecting PKC phosphorylation. The inhibitory effects of prednisolone on MCP-1 expression were reversed by mifepristone, a glucocorticoid receptor antagonist. Our results indicate that glucose induces MCP-1 mainly through hyperosmolarity by activating PKC and its downstream NF-kappaB, and that such effect was inhibited by prednisolone, suggesting the efficacy of prednisolone in preventing peritoneal fibrosis in patients on CAPD.
Mesh Headings (Keywords): Animals, Blotting, Western, Cells, Cultured, Chemokine CCL2, Enzyme Activation, Epithelium, Fibrosis, Gene Expression Regulation, Glucose, I-kappa B Proteins, Mifepristone, Mitogen-Activated Protein Kinases, NF-kappa B, Osmolar Concentration, Peritoneal Dialysis, Continuous Ambulatory, Peritoneum, Phosphorylation, Prednisolone, Protein Kinase C, RNA, Messenger, Rats
Check for Full Text / PubMed Unique Identifier (PMID): 16518329
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