Rapid Release of Active Tissue Factor from Human Arterial Smooth Muscle Cells Under Flow Conditions.
From: Institut für Pharmakologie und Klinische Pharmakologie, Universitätsklinikum Düsseldorf, Germany.
Arteriosclerosis, thrombosis, and vascular biology
- Publish Date: May 2006
- ISSN: 1524-4636
- Volume: 26
- Issue: 5
- Pages: e34-7
- Medium: Internet
- Language: English
- Citation (JAMA): Stampfuss Jan-Julius, Censarek Petra, Fischer Jens W, et al. Rapid Release of Active Tissue Factor from Human Arterial Smooth Muscle Cells Under Flow Conditions.. Arterioscler. Thromb. Vasc. Biol. May 2006;26:e34-7
Abstract
Circulating tissue factor (TF) is an important determinant of coronary thrombosis. Among other cell types, such as monocytes, vascular smooth muscle cells (SMCs) are capable of releasing TF. When studied under static conditions, SMCs do release TF, but this process is slow and, thus, cannot explain the elevated levels of circulating TF, as observed in patients with acute coronary syndromes. The present study demonstrates that cultured human mammary artery SMCs very rapidly (minutes) release active, microparticle-bound TF when exposed to flow conditions. There was a clear log-linear correlation between the shear rate (range 10 s(-1) to 1500 s(-1)) and the procoagulant activity of SMC perfusates. Flow-dependent release of TF was transient (10 minutes) and did not measurably reduce cell surface TF content. Interestingly, a time-dependent (t(1/2) 30 minutes) re-exposure of releasable TF was detected after a no-flow period. These data demonstrate that SMCs may become a pathophysiologically relevant source of TF that can be rapidly released into the circulation in situations in which endothelial damage occurs and SMCs come into a close contact with the flowing blood.
Mesh Headings (Keywords): Blood Circulation, Cells, Cultured, Humans, Mammary Arteries, Muscle, Smooth, Vascular, Myocytes, Smooth Muscle, Thromboplastin
Check for Full Text / PubMed Unique Identifier (PMID): 16528008
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