Double-stranded Rna is Internalized by Scavenger Receptor-mediated Endocytosis in Drosophila S2 Cells.
From: Department of Pediatrics and Biocenter Oulu, University of Oulu, FIN-90014, Oulu, Finland.
The Journal of biological chemistry
- Publish Date: May 2006
- ISSN: 0021-9258
- Volume: 281
- Issue: 20
- Pages: 14370-5
- Medium: Print
- Language: English
- Citation (JAMA): Ulvila Johanna, Parikka Mataleena, Kleino Anni, et al. Double-stranded Rna is Internalized by Scavenger Receptor-mediated Endocytosis in Drosophila S2 Cells.. J. Biol. Chem. May 2006;281:14370-5
Abstract
Double-stranded RNA (dsRNA) fragments are readily internalized and processed by Drosophila S2 cells, making these cells a widely used tool for the analysis of gene function by gene silencing through RNA interference (RNAi). The underlying mechanisms are insufficiently understood. To identify components of the RNAi pathway in S2 cells, we developed a screen based on rescue from RNAi-induced lethality. We identified Argonaute 2, a core component of the RNAi machinery, and three gene products previously unknown to be involved in RNAi in Drosophila: DEAD-box RNA helicase Belle, 26 S proteasome regulatory subunit 8 (Pros45), and clathrin heavy chain, a component of the endocytic machinery. Blocking endocytosis in S2 cells impaired RNAi, suggesting that dsRNA fragments are internalized by receptor-mediated endocytosis. Indeed, using a candidate gene approach, we identified two Drosophila scavenger receptors, SR-CI and Eater, which together accounted for more than 90% of the dsRNA uptake into S2 cells. When expressed in mammalian cells, SR-CI was sufficient to mediate internalization of dsRNA fragments. Our data provide insight into the mechanism of dsRNA internalization by Drosophila cells. These results have implications for dsRNA delivery into mammalian cells.
Mesh Headings (Keywords): Animals, Cell Line, Drosophila melanogaster, Endocytosis, Gene Library, Gene Silencing, Genetic Techniques, Luciferases, RNA Interference, RNA, Double-Stranded, Receptors, Scavenger
Check for Full Text / PubMed Unique Identifier (PMID): 16531407
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.