Plasma Membrane Domains Specialized for Clathrin-mediated Endocytosis in Primary Cells.
From: Department of Physiology, University of Massachusetts Medical School, Worcester 01605, USA.
The Journal of biological chemistry
- Publish Date: Jun 2006
- ISSN: 0021-9258
- Volume: 281
- Issue: 23
- Pages: 16139-46
- Medium: Print
- Language: English
- Citation (JAMA): Bellve Karl D, Leonard Deborah, Standley Clive, et al. Plasma Membrane Domains Specialized for Clathrin-mediated Endocytosis in Primary Cells.. J. Biol. Chem. Jun 2006;281:16139-46
Abstract
Clathrin assembly at the plasma membrane is a fundamental process required for endocytosis. In cultured cells, most of the clathrin is localized to large patches that display little lateral mobility. The functional role of these regions is not clear, and it has been thought that they may represent artifacts of cell adhesion of cultured cells. Here we have analyzed clathrin organization in primary adipose cells isolated from mice, which are nonadherent and fully differentiated. The majority of clathrin on the plasma membrane of these cells (>60%) was found in large clathrin patches that displayed virtually no lateral mobility and persisted for many minutes, and a smaller amount was found in small spots that appeared and disappeared rapidly. Direct visualization of transferrin revealed that it bound onto large arrays of clathrin, internalizing through vesicles that emerge from these domains. High resolution imaging (50 images/s) revealed fluorescence intensity fluctuations consistent with the formation and detachment of coated vesicles from within large patches. These results reveal that large clathrin assemblies are active regions of endocytosis in mammalian cells and highlight the importance of understanding the mechanistic basis for this organization.
Mesh Headings (Keywords): 3T3-L1 Cells, Animals, Cells, Cultured, Clathrin, Endocytosis, Fluorescent Dyes, Male, Mice, Mice, Inbred C57BL
Check for Full Text / PubMed Unique Identifier (PMID): 16537543
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