Role for Centromeric Heterochromatin and Pml Nuclear Bodies in the Cellular Response to Foreign Dna.
From: MRC Clinical Sciences Centre, Imperial College London, Hammersmith Hospital Campus, Du Cane Road, London W12 0NN, United Kingdom.
Molecular and cellular biology
- Publish Date: Apr 2006
- ISSN: 0270-7306
- Volume: 26
- Issue: 7
- Pages: 2583-94
- Medium: Print
- Language: English
- Citation (JAMA): Bishop Cleo L, Ramalho Michal, Nadkarni Nachiket, et al. Role for Centromeric Heterochromatin and Pml Nuclear Bodies in the Cellular Response to Foreign Dna.. Mol. Cell. Biol. Apr 2006;26:2583-94
Abstract
Nuclear spatial positioning plays an important role in the epigenetic regulation of eukaryotic gene expression. Here we show a role for nuclear spatial positioning in regulating episomal transgenes that are delivered by virus-like particles (VLPs). VLPs mediate the delivery of plasmid DNA (pDNA) to cell nuclei but lack viral factors involved in initiating and regulating transcription. By tracking single fluorescently labeled VLPs, coupled with luciferase reporter gene assays, we found that VLPs transported pDNA to cell nuclei efficiently but transgenes were immediately silenced by the cell. An investigation of the nuclear location of fluorescent VLPs revealed that the pDNAs were positioned next to centromeric heterochromatin. The activation of transcription by providing viral factors or inhibiting histone deacetylase activity resulted in the localization to euchromatin regions. Further, the activation of transcription induced the recruitment of PML nuclear bodies (PML-NBs) to the VLPs. This association did not play a role in regulating transgene expression, but PML protein was necessary for the inhibition of transgene expression with alpha interferon (IFN-alpha). These results support a model whereby cells can prevent foreign gene expression at two levels: by positioning transgenes next to centromeric heterochromatin or, if that is overcome, via the type I IFN response facilitated by PML-NB recruitment.
Mesh Headings (Keywords): Animals, COS Cells, Cell Nucleus Structures, Cells, Cultured, Centromere, Cercopithecus aethiops, DNA, Gene Silencing, Gene Transfer Techniques, Hela Cells, Heterochromatin, Humans, Hydroxamic Acids, Immunity, Cellular, Mice, Plasmids, Swiss 3T3 Cells, Transcription, Genetic, Transgenes, Virus Replication
Check for Full Text / PubMed Unique Identifier (PMID): 16537904
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