Cyclooxygenase-2 Rescues Rat Mesangial Cells from Apoptosis Induced by Adriamycin Via Upregulation of Multidrug Resistance Protein 1 (P-glycoprotein).
From: Department of Medicine, Cardiovascular Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road, Milwaukee, WI 53226, USA.
Journal of the American Society of Nephrology : JASN
- Publish Date: Apr 2006
- ISSN: 1046-6673
- Volume: 17
- Issue: 4
- Pages: 977-85
- Medium: Print
- Language: English
- Citation (JAMA): Miller Bradley, Patel Vimal A, Sorokin Andrey, et al. Cyclooxygenase-2 Rescues Rat Mesangial Cells from Apoptosis Induced by Adriamycin Via Upregulation of Multidrug Resistance Protein 1 (P-glycoprotein).. J. Am. Soc. Nephrol. Apr 2006;17:977-85
Abstract
Cyclooxygenase-2 (COX-2) is constitutively expressed in restricted subpopulations of kidney cells, where it presumably acts as an antiapoptotic factor. In conditions that are characterized by inflammation, COX-2 expression also has been described in glomerular mesangial cells (GMC), where COX-2 is not expressed constitutively. It was shown previously that adenovirus-mediated gene transfer of COX-2 into rat GMC led to increased expression and activity of multidrug resistance protein 1 (MDR-1), a membrane transporter that functions as an efflux pump for chemotherapeutic drugs, including Adriamycin (ADR). In ADR nephrotoxicity, a pathologic change in glomeruli could be partially explained by ADR-mediated changes in GMC. Here it is demonstrated that ADR (also known as doxorubicin; 1 microg/ml) induced apoptosis in 15.3 +/- 2.2% of GMC, whereas after adenovirus-mediated COX-2 expression, only 6.6 +/- 0.4% of ADR-treated cells underwent apoptosis. This protective effect was nullified by treatment with NS398, specific COX-2 inhibitor. ADR efflux is greater in COX-2-overexpressing cells, when compared with control, which is attributed to the increased MDR-1 expression. Addition of PSC833, the specific MDR-1 inhibitor, completely abolished the protective effect of COX-2 overexpression and increased the level of apoptosis in GMC that were exposed to ADR. These data suggest that COX-2 protects GMC from ADR-mediated apoptosis via transcriptional upregulation of MDR-1 and that induced COX-2 expression would lessen ADR nephrotoxicity.
Mesh Headings (Keywords): Animals, Apoptosis, Cells, Cultured, Cyclooxygenase 2, Cyclosporins, Doxorubicin, Glomerular Mesangium, P-Glycoprotein, P-Glycoproteins, Rats, Up-Regulation
Check for Full Text / PubMed Unique Identifier (PMID): 16540558
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