Genetic Manipulation of Intraspinal Plasticity After Spinal Cord Injury Alters the Severity of Autonomic Dysreflexia.
From: Spinal Cord and Brain Injury Research Center, University of Kentucky, Lexington, Kentucky 40536, USA.
The Journal of neuroscience : the official journal of the Society for Neuroscience
- Publish Date: Mar 2006
- ISSN: 1529-2401
- Volume: 26
- Issue: 11
- Pages: 2923-32
- Medium: Internet
- Language: English
- Citation (JAMA): Cameron Adrian A, Smith George M, Randall David C, et al. Genetic Manipulation of Intraspinal Plasticity After Spinal Cord Injury Alters the Severity of Autonomic Dysreflexia.. J. Neurosci. Mar 2006;26:2923-32
Abstract
Severe spinal cord injuries above mid-thoracic levels can lead to a potentially life-threatening hypertensive condition termed autonomic dysreflexia, which is often triggered by painful distension of pelvic viscera (bladder or bowel) and consequent sensory fiber activation, including nociceptive C-fibers. Interruption of tonically active medullo-spinal pathways after injury causes disinhibition of thoracolumbar sympathetic preganglionic neurons, and intraspinal sprouting of nerve growth factor (NGF)-responsive primary afferent fibers is thought to contribute to their hyperactivity. We investigated spinal levels that are critical for eliciting autonomic dysreflexia using a model of noxious colorectal distension (CRD) after complete spinal transection at the fourth thoracic segment in rats. Post-traumatic sprouting of calcitonin gene-related peptide (CGRP)-immunoreactive primary afferent fibers was selectively altered at specific spinal levels caudal to the injury with bilateral microinjections of adenovirus encoding the growth-promoting NGF or growth-inhibitory semaphorin 3A (Sema3a) compared with control green fluorescent protein (GFP). Two weeks later, cardio-physiological responses to CRD were assessed among treatment groups before histological analysis of afferent fiber density at the injection sites. Dysreflexic hypertension was significantly higher with NGF overexpression in lumbosacral segments compared with GFP, whereas similar overexpression of Sema3a significantly reduced noxious CRD-evoked hypertension. Quantitative analysis of CGRP immunostaining in the spinal dorsal horns showed a significant correlation between the extent of fiber sprouting into the spinal segments injected and the severity of autonomic dysreflexia. These results demonstrate that site-directed genetic manipulation of axon guidance molecules after complete spinal cord injury can alter endogenous circuitry to modulate plasticity-induced autonomic pathophysiology.
Mesh Headings (Keywords): Adenoviridae, Afferent Pathways, Animals, Autonomic Dysreflexia, Autonomic Fibers, Preganglionic, Axons, Bradycardia, Calcitonin Gene-Related Peptide, Colon, Cordotomy, Dilatation, Pathologic, Disease Models, Animal, Female, Gene Therapy, Genetic Vectors, Hypertension, Microinjections, Nerve Growth Factor, Neuronal Plasticity, Pain, Posterior Horn Cells, Rats, Rats, Wistar, Recombinant Fusion Proteins, Rectum, Semaphorin-3A, Spinal Cord Injuries, Substance P, Vasoconstriction
Check for Full Text / PubMed Unique Identifier (PMID): 16540569
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