Fgf-2 Deficiency Does Not Alter Vulnerability of the Dopaminergic Nigrostriatal System Towards Mptp Intoxication in Mice.
From: Interdisciplinary Center for Neurosciences (IZN), Department of Neuroanatomy, University of Heidelberg, Im Neuenheimer Feld 307, D-69120 Heidelberg, Germany.
The European journal of neuroscience
- Publish Date: Mar 2006
- ISSN: 0953-816X
- Volume: 23
- Issue: 6
- Pages: 1671-5
- Medium: Print
- Language: English
- Citation (JAMA): Zechel S, Jarosik J, Kiprianova I, et al. Fgf-2 Deficiency Does Not Alter Vulnerability of the Dopaminergic Nigrostriatal System Towards Mptp Intoxication in Mice.. Eur. J. Neurosci. Mar 2006;23:1671-5
Abstract
Fibroblast growth factor 2 (FGF-2) was the first growth factor discovered that exerted prominent protective and regenerative effects in an animal model of Parkinson’s disease, the MPTP-lesioned dopaminergic nigrostriatal system. To address the putative physiological relevance of endogenous FGF-2 for midbrain dopaminergic neurons, we have analysed densities of tyrosine hydroxylase (TH)-positive cells in the substantia nigra (SN) and TH-positive fibers in the striatum and amygdala of adult FGF-2-deficient mice. We found that densities of TH-immunoreactive (ir) cells in the SN as well as densities of TH-ir fibers in the striatum and amygdala were unaltered as compared with wild-type littermates. There is evidence to suggest that growth factor deficits do not become apparent unless a system is challenged in a lesioning paradigm. We therefore tested the ability of the nigrostriatal system with respect to its ability to cope with MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine) intoxication. Treatment with 20 mg/kg MPTP on three consecutive days reduced dopamine levels in the striatum by about 80%. Densities of TH-positive neurons in the SN were reduced by 71%. However, both parameters did not significantly differ between FGF-2(-/-) mice and wild-type littermates. Our results therefore suggest that FGF-2, despite its prominent pharmacological potency as a neurotrophic factor for the dopaminergic nigrostriatal system, is not crucial for maintaining its structural integrity and ability to cope with MPTP intoxication.
Mesh Headings (Keywords): 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine, Amygdala, Animals, Axons, Dopamine, Dopamine Agents, Fibroblast Growth Factor 2, Immunohistochemistry, Mice, Mice, Knockout, Neostriatum, Substantia Nigra, Tyrosine 3-Monooxygenase
Check for Full Text / PubMed Unique Identifier (PMID): 16553632
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