The Kinesin Kif1c and Microtubule Plus Ends Regulate Podosome Dynamics in Macrophages.
From: Institut für Prophylaxe und Epidemiologie der Kreislaufkrankheiten, Ludwig-Maximilians-Universität, 80336 Munich, Germany.
Molecular biology of the cell
- Publish Date: Jun 2006
- ISSN: 1059-1524
- Volume: 17
- Issue: 6
- Pages: 2811-23
- Medium: Print
- Language: English
- Citation (JAMA): Kopp Petra, Lammers Reiner, Aepfelbacher Martin, et al. The Kinesin Kif1c and Microtubule Plus Ends Regulate Podosome Dynamics in Macrophages.. Mol. Biol. Cell Jun 2006;17:2811-23
Abstract
Microtubules are important for the turnover of podosomes, dynamic, actin-rich adhesions implicated in migration and invasion of monocytic cells. The molecular basis for this functional dependency, however, remained unclear. Here, we show that contact by microtubule plus ends critically influences the cellular fate of podosomes in primary human macrophages. In particular, we identify the kinesin KIF1C, a member of the Kinesin-3 family, as a plus-end-enriched motor that targets regions of podosome turnover. Expression of mutation constructs or small interfering RNA-/short hairpin RNA-based depletion of KIF1C resulted in decreased podosome dynamics and ultimately in podosome deficiency. Importantly, protein interaction studies showed that KIF1C binds to nonmuscle myosin IIA via its PTPD-binding domain, thus providing an interface between the actin and tubulin cytoskeletons, which may facilitate the subcellular targeting of podosomes by microtubules. This is the first report to implicate a kinesin in podosome regulation and also the first to describe a function for KIF1C in human cells.
Mesh Headings (Keywords): Cell Differentiation, Cell Membrane Structures, Cells, Cultured, Cloning, Molecular, Escherichia coli, Humans, Kinesin, Macrophages, Microinjections, Microtubules, Mutagenesis, Plasmids, RNA, Small Interfering, Reverse Transcriptase Polymerase Chain Reaction, Transfection
Check for Full Text / PubMed Unique Identifier (PMID): 16554367
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.