15-methoxypinusolidic Acid from Biota Orientalis Attenuates Glutamate-induced Neurotoxicity in Primary Cultured Rat Cortical Cells.
From: College of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, San 56-1, Shillim-Dong, Gwanak-Gu, Seoul 151-742, Republic of Korea.
Toxicology in vitro : an international journal published in association with BIBRA
- Publish Date: Sep 2006
- ISSN: 0887-2333
- Volume: 20
- Issue: 6
- Pages: 936-41
- Medium: Print
- Language: English
- Citation (JAMA): Koo Kyung Ah, Kim Seung Hyun, Lee Mi Kyeong, et al. 15-methoxypinusolidic Acid from Biota Orientalis Attenuates Glutamate-induced Neurotoxicity in Primary Cultured Rat Cortical Cells.. Sep 2006;20:936-41
Abstract
15-Methoxypinusolidic acid (15-MPA), a pinusolide derivative isolated from Biota orientalis (Cupressaceae) leaves prevented glutamate-induced excitotoxicity in primary cultured rat cortical cells in vitro. 15-MPA had more selectivity in protecting neurons against N-methyl-D-aspartate (NMDA)-induced neurotoxicity than that induced by kainic acid (KA). The glutamate-induced increase of intracellular calcium ([Ca2+]i) in cortical cells was effectively reduced by 15-MPA. Moreover, 15-MPA could successfully reduce the subsequent overproduction of nitric oxide (NO) and the level of cellular peroxide, and inhibit glutathione (GSH) depletion and lipid peroxidation induced by glutamate in our cultures. Collectively, these results suggested that 15-MPA attenuated glutamate-induced excitotoxicity via stabilization of [Ca2+]i homeostasis and suppression of oxidative stress possibly through the actions on the NMDA receptors.
Mesh Headings (Keywords): Animals, Calcium, Cells, Cultured, Cerebral Cortex, Diterpenes, Glutamic Acid, Glutathione, Lipid Peroxidation, Neuroprotective Agents, Nitric Oxide, Oxidative Stress, Rats, Rats, Sprague-Dawley, Thuja
Check for Full Text / PubMed Unique Identifier (PMID): 16564156
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