Relative Activity of a Tobacco Hybrid Expressing High Levels of a Tobacco Anionic Peroxidase and Maize Ribosome-inactivating Protein Against Helicoverpa Zea and Lasioderma Serricorne.
From: Crop BioProtection Research Unit, National Center for Agricultural Utilization Research, Agricultural Research Service, U.S. Department of Agriculture, 1815 North University Street, Peoria, Illinois 61604, USA. dowdpf@ncaur.usda.gov
Journal of agricultural and food chemistry
- Publish Date: Apr 2006
- ISSN: 0021-8561
- Volume: 54
- Issue: 7
- Pages: 2629-34
- Medium: Print
- Language: English
- Citation (JAMA): Dowd Patrick F, Holmes Robert A, Pinkerton T Scott, et al. Relative Activity of a Tobacco Hybrid Expressing High Levels of a Tobacco Anionic Peroxidase and Maize Ribosome-inactivating Protein Against Helicoverpa Zea and Lasioderma Serricorne.. J. Agric. Food Chem. Apr 2006;54:2629-34
Abstract
Tobacco (Nicotiana tabacum) plants grown from seed obtained by crossing a tobacco line that expressed an activated maize ribosome-inactivating protein (RIP) with a line that overexpressed tobacco anionic peroxidase were tested for their effects on corn earworm Helicoverpa zea and cigarette beetle Lasioderma serricorne larvae as compared to the wild-type plant cross. Significant feeding reductions were noted for transgenic plants expressing both resistance proteins as compared to wild-type plants for both H. zea and L. serricorne. Significant increases in mortality were also noted for those insects fed on the transgenic cross as compared to wild-type plants in some cases. Levels of both peroxidase and maize RIP were significantly higher in transgenic as compared to wild-type plants (which did not produce maize RIP). The degree of feeding was significantly negatively correlated with the level of RIP or peroxidase individually.
Mesh Headings (Keywords): Animals, Beetles, Lepidoptera, Peroxidases, Pest Control, Biological, Plant Proteins, Plants, Genetically Modified, Ribosomes, Tobacco, Zea mays
Check for Full Text / PubMed Unique Identifier (PMID): 16569054
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