In Vitro Evaluation of the Effect of a Novel Immunosuppressive Agent, Fty720, on the Function of Feline Neutrophils.
From: Comparative Transplantation Laboratory, Department of Surgical and Radiological Sciences and Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis, CA 95616-8745, USA.
American journal of veterinary research
- Publish Date: Apr 2006
- ISSN: 0002-9645
- Volume: 67
- Issue: 4
- Pages: 588-92
- Medium: Print
- Language: English
- Citation (JAMA): Chen Yi-Je, Kyles Andrew E, Gregory Clare R, et al. In Vitro Evaluation of the Effect of a Novel Immunosuppressive Agent, Fty720, on the Function of Feline Neutrophils.. Am. J. Vet. Res. Apr 2006;67:588-92
Abstract
OBJECTIVE: To use in vitro assays to evaluate the effects of a novel immunosuppressive agent, FTY720, on biological functions (migration, phagocytosis, and production of reactive-oxygen species [ROS]) of feline peripheral neutrophils and determine the cytotoxic effects of FTY720 on feline peripheral neutrophils. SAMPLE POPULATION: Peripheral neutrophils obtained from 8 healthy cats. PROCEDURE: Peripheral neutrophils were isolated from blood samples obtained from the 8 cats and exposed to the phosphorylated form of FTY720 (FTY720-P). A fluorescence-based in vitro evaluation of migration was performed. Phagocytosis of microbes and production of ROS were evaluated by use of a 2-color flow cytometry system. Samples of whole blood obtained from the cats were incubated with various concentrations of FTY720-P, fluorescein-labeled Staphylococcus aureus, and dihydroethidium. Cytotoxic effects were evaluated by use of propidium iodide staining. RESULTS: Addition of FTY720-P caused a slight non-significant decrease in phagocytosis and production of ROS by feline peripheral neutrophils. Migration activity of feline peripheral neutrophils was significantly increased by the addition of FTY720-P. Addition of FTY720-P at concentrations considered for clinical use did not increase the death rate of feline peripheral neutrophils. CONCLUSIONS AND CLINICAL RELEVANCE: FTY720 does not inhibit critical functions of feline peripheral neutrophils in vitro.
Mesh Headings (Keywords): Animals, Cats, Immunosuppressive Agents, Leukocyte Count, Neutrophils, Phosphorylation, Propylene Glycols, Reactive Oxygen Species, Sphingosine
Check for Full Text / PubMed Unique Identifier (PMID): 16579750
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