Mucosal Genetic Immunization Against Four Adhesins Protects Against Staphylococcus Aureus-induced Mastitis in Mice.
From: Department of Histology, Microbiology and Medical Biotechnologies, University of Padua, Via Gabelli 63, Padova, Italy.
Vaccine
- Publish Date: May 2006
- ISSN: 0264-410X
- Volume: 24
- Issue: 20
- Pages: 4393-402
- Medium: Print
- Language: English
- Citation (JAMA): Castagliuolo Ignazio, Piccinini Renata, Beggiao Elisa, et al. Mucosal Genetic Immunization Against Four Adhesins Protects Against Staphylococcus Aureus-induced Mastitis in Mice.. Vaccine May 2006;24:4393-402
Abstract
Staphylococcus aureus is the most common etiologic agent of mastitis in bovines and a major cause of economic losses in the dairy industry. Since adhesins, that anchor bacteria to the extracellular matrix (ECM), are among the most important S. aureus virulence factors, we used four adhesion factors (fibrinogen binding protein, fibronectin binding protein A, clumping factor A and collagen adhesin) as target in a DNA vaccine. Intranasal immunization with a pDNA mixture coding the four adhesins, triggered significant levels of specific serum and mucosal Ig that inhibited S. aureus adhesion to cow mammary gland epithelial cells in vitro. Splenocytes of immunized mice challenged in vitro with S. aureus extracts showed a strong proliferative response. Finally, immunized mice were significantly protected against intramammary challenge with S. aureus. Our data confirm the feasibility of a genetic mucosal vaccine targeting S. aureus adhesins and prompt further study to test the efficacy in preventing cow mastitis.
Mesh Headings (Keywords): Adhesins, Bacterial, Animals, Antibodies, Bacterial, Base Sequence, Cell Proliferation, DNA Primers, Enzyme-Linked Immunosorbent Assay, Female, Immunity, Mucosal, Mammary Glands, Animal, Mastitis, Mice, Mice, Inbred BALB C, Spleen, Staphylococcal Infections, Staphylococcus aureus
Check for Full Text / PubMed Unique Identifier (PMID): 16580097
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