Fluorogold Labeling of Descending Brain Neurons in Larval Lamprey Does Not Cause Cell Death.
From: Division of Biological Sciences and Interdisciplinary Neuroscience Program, 114 Lefevre Hall, University of Missouri, Columbia, MO 65211-6190, USA.
Neuroscience letters
- Publish Date: Jun 2006
- ISSN: 0304-3940
- Volume: 401
- Issue: 1-2
- Pages: 119-24
- Medium: Print
- Language: English
- Citation (JAMA): McClellan Andrew D, Zhang Lei, Palmer Ryan, et al. Fluorogold Labeling of Descending Brain Neurons in Larval Lamprey Does Not Cause Cell Death.. Neurosci. Lett. Jun 2006;401:119-24
Abstract
In our previous double-labeling studies, the fluorescent anatomical tracers Fluorogold (FG) and Texas red dextran amine (TRDA) were used to demonstrate that descending brain neurons, approximately 80% of which are reticulospinal (RS) neurons, in spinal cord-transected larval lamprey regenerate their axons. However, the numbers of FG-labeled descending brain neurons decreased significantly with increasing recovery times, from 2 to 16 weeks. For some FG-labeled mammalian neurons, FG appears to degrade and/or be lost over time, while in other neurons this tracer can kill neurons. In the present study, these possibilities were examined in larval lamprey for FG-labeled descending brain neurons. As in our previous studies, FG was applied to the spinal cord at 40% body length (BL, relative distance from the head) to retrogradely labeled descending brain neurons, and after recovery times of 2, 8, or 16 weeks, HRP, a non-toxic retrograde tracer, was applied to the spinal cord at 20% BL to determine if the numbers of HRP-labeled neurons were reduced. At these three recovery times, the numbers of HRP-labeled descending brain neurons were not significantly different than the numbers of HRP-labeled neurons in control animals that were not labeled with FG. Furthermore, the size and morphology of cell bodies and dendritic trees were not noticeably different in descending brain neurons with and without FG. Thus, in larval lamprey, FG does not appear to kill these neurons, but some FG probably is degraded and/or lost from neurons with increasing recovery times.
Mesh Headings (Keywords): Animals, Brain Stem, Cell Count, Cell Death, Efferent Pathways, Fluorescent Dyes, Growth Cones, Horseradish Peroxidase, Lampreys, Larva, Nerve Degeneration, Nerve Regeneration, Neurotoxins, Reticular Formation, Spinal Cord Injuries, Staining and Labeling, Stilbamidines, Time Factors
Check for Full Text / PubMed Unique Identifier (PMID): 16580134
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.