Strength of Multiple Parallel Biological Bonds.
From: Physical Biosciences Institute, Chemistry and Materials Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, California 94550, USA.
Biophysical journal
- Publish Date: Jun 2006
- ISSN: 0006-3495
- Volume: 90
- Issue: 12
- Pages: 4686-91
- Medium: Print
- Language: English
- Citation (JAMA): Sulchek Todd, Friddle Raymond W, Noy Aleksandr, et al. Strength of Multiple Parallel Biological Bonds.. Biophys. J. Jun 2006;90:4686-91
Abstract
Multivalent interactions play a critical role in a variety of biological processes on both molecular and cellular levels. We have used molecular force spectroscopy to investigate the strength of multiple parallel peptide-antibody bonds using a system that allowed us to determine the rupture forces and the number of ruptured bonds independently. In our experiments the interacting molecules were attached to the surfaces of the probe and sample of the atomic force microscope with flexible polymer tethers, and the unique mechanical signatures of the tethers determined the number of ruptured bonds. We show that the rupture forces increase with the number of interacting molecules and that the measured forces obey the predictions of a Markovian model for the strength of multiple parallel bonds. We also discuss the implications of our results to the interpretation of force spectroscopy measurements in multiple bond systems.
Mesh Headings (Keywords): Antibodies, Antigen-Antibody Complex, Binding Sites, CA-15-3 Antigen, Computer Simulation, Microscopy, Atomic Force, Models, Chemical, Models, Molecular, Peptide Fragments, Protein Binding
Check for Full Text / PubMed Unique Identifier (PMID): 16581843
This abstract is part of PubMed, a service of the U.S. National Library of Medicine. PubMed includes more than 17 million citations from MEDLINE and other life science journals for biomedical articles. See Copyright and Disclaimers.
Linked medical terms appearing on this page are added by Healia to help readers find more information and are not part of the original PubMed document.
The data herein was last updated on July 8th, 2008 and may not reflect the most current and accurate data available from NLM.